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Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112127 pLL1 sgRNA(MS2)-MS2-AIDmono empty sgRNA cloning vector with MS2-AIDmono
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112128 pLL2 sgRNA(MS2)-MS2-AID3c empty sgRNA cloning vector with MS2-AID3c
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112129 pLL3 sgRNA(MS2)-MS2-AID3f empty sgRNA cloning vector with MS2-AID3f
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112130 pLL4 sgRNA(MS2)-MS2-hA3a empty sgRNA cloning vector with MS2-humanA3a
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112131 pLL5 sgRNA(MS2)-MS2-hA3a D131A empty sgRNA cloning vector with MS2-humanA3a D131A
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112132 pLL6 sgRNA(MS2)-MS2-hA3a D131E empty sgRNA cloning vector with MS2-humanA3a D131E
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112133 pLL7 lenti-dcas9-3xflag-blast lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
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112134 pLL8 lenti-D10Acas9-3xflag-blast lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)
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