Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed Article
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 53186 | phH1-gRNA | Expresses the S. pyogenes sgRNA from the H1 promoter |
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| 53187 | pmU6-gRNA | Expresses the S. pyogenes sgRNA from the mouse U6 promoter |
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| 53188 | phU6-gRNA | Expresses the S. pyogenes sgRNA from the human U6 promoter |
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| 53189 | ph7SK-gRNA | Expresses the S. pyogenes sgRNA from the 7SK promoter |
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| 53190 | pLV hUbC-Cas9-T2A-GFP | 3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP |
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| 53191 | pLV hUbC-dCas9-T2A-GFP | Co-expresses human optimized S. pyogenes dCas9 and GFP |
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| 53192 | pLV hUbC-dCas9 VP64-T2A-GFP | Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP |
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| 59791 | pLV hUbC-VP64 dCas9 VP64-T2A-GFP | Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP |
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| 67620 | pLV hUbC-dCas9 KRAB-T2A-GFP | Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP |
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| 84034 | pLV GG hUbC-dsRED | Contains Golden Gate cassette to express up to four gRNAs and dsRED |
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