|
pdCas9-humanized |
dead Cas9 with 3X NLS (Homo sapiens) |
MSCV LTR promoter |
Puromycin |
Qi
|
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
|
Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
pMSCVpuro
|
|
pdCas9::BFP-humanized |
dCas9 fused to BFP (Homo sapiens) |
MSCV LTR promoter |
Puromycin |
Qi
|
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
|
Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
pMSCVpuro
|
|
pHR-SFFV-dCas9-BFP |
dCas9-BFP fusion (Homo sapiens) |
SFFV |
|
Weissman
|
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
|
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP
pHR
|
|
pHR-SFFV-dCas9-BFP-KRAB |
dCas9-BFP-KRAB fusion (Homo sapiens) |
SFFV |
|
Weissman
|
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
|
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
pHR
|
|
pcDNA-dCas9 |
dCas9 (Other) |
CMV |
|
Gersbach
|
RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
|
Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
pcDNA3.1
|
|
Cas9m2 |
Cas9m2 (Other) |
|
|
Church
|
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+H840A
pcDNA3.3_TOPO
|
|
Cas9m3 |
Cas9m3 (Other) |
|
|
Church
|
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+D839A+H840A
pcDNA3.3_TOPO
|
|
Cas9m4 |
Cas9m4 (Other) |
|
|
Church
|
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
|
Cas9 D10A+D839A+H840A+N863A
pcDNA3.3_TOPO
|
|
pHAGE TRE dCas9 |
dCas9 (Other) |
TRE |
Neomycin (select with G418) |
Wolfe
|
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Tet-regulatable dCas9 lentiviral expression vector
pHAGE
|
|
pHAGE TRE dCas9-KRAB |
dCas9 (Other) |
TRE |
Neomycin (select with G418) |
Wolfe
|
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector
pHAGE
|
|
pHAGE EF1α dCas9-KRAB |
dCas9 (Other) |
EF1alpha |
Puromycin |
Wolfe
|
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
|
Constitutive dCas9-KRAB 2nd generation lentiviral expression vector
pHAGE
|
|
pSLQ1658-dCas9-EGFP |
dCas9 fuse to EGFP (Homo sapiens) |
MSCV LTR promoter |
Puromycin |
Qi
|
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.
Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
|
Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)
pMSCVpuro
|
|
pLV hUbC-dCas9-T2A-GFP |
humanized dead Cas9 T2A GFP (Other) |
hUbC |
Zeo marker is outside the LTRs and will not be packaged into virus. |
Gersbach
|
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
|
Co-expresses human optimized S. pyogenes dCas9 and GFP
FUGW
|
|
pcDNA3.1-CibN-dCas9 |
CibN-dCas9 (Arabidopsis thaliana) |
CMV |
Neomycin (select with G418) |
Gersbach
|
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses CibN-dCas9 in mammalian cells
pcDNA3.1
|
|
pcDNA3.1-dCas9-CibN |
dCas9-CibN (Arabidopsis thaliana) |
CMV |
Neomycin (select with G418) |
Gersbach
|
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses dCas9-CibN in mammalian cells
pcDNA3.1
|
|
pcDNA3.1-CibN-dCas9-CibN |
dCas9-CibN (Arabidopsis thaliana) |
CMV |
Neomycin (select with G418) |
Gersbach
|
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
|
Expresses CibN-dCas9-CibN in mammalian cells
pcDNA3.1
|
|
pHR-SFFV-KRAB-dCas9-P2A-mCherry |
KRAB-dCas9-P2A-mCherry fusion (Homo sapiens) |
SFFV |
mCherry |
Weissman
|
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.
|
2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry
pHR
|
|
pcDNA-dCas9-HA |
S.pyogenes dCas9 with c-terminal HA tag (Synthetic) |
CMV |
|
Gersbach
|
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
pcDNA3.1
|
|
pX330A_dCas9-1x2 |
humanized S. pyogenes dCas9 (Other) |
CBh |
|
Yamamoto
|
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
|
pX330A_dCas9-1x3 |
humanized S. pyogenes dCas9 (Other) |
CBh |
|
Yamamoto
|
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
|
pX330A_dCas9-1x5 |
humanized S. pyogenes dCas9 (Other) |
CBh |
|
Yamamoto
|
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
|
pX330A_dCas9-1x6 |
humanized S. pyogenes dCas9 (Other) |
CBh |
|
Yamamoto
|
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
BMC Biotechnol. 2015 May 22;15(1):33.
|
Expresses dCas9 and gRNA
pUC ori vector
|
|
pEF1a-CRY2PHR-SID4X |
SID4X-CYR2PHR (Synthetic) |
EF1alpha |
|
Kong
|
Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer
Theranostics
|
AAV expression of CRYS2PHR-SID4X
pAAV
|
|
px330-CIB1-dCas9 |
|
U6 and CMV |
|
Kong
|
Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer
Theranostics
|
Mammalian gRNA expression vector also expressing CIB1-dCas9
px330
|
|
pCCL-PGK-SPdCas9-BFP-DNMT1 |
dCas9-BFP-DNMT1, catalytic domain |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT1 catalytic domain
3rd Generation lentiviral vector
|
|
pCCL-PGK-SPdCas9-BFP-DNMT3A |
dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3A catalytic domain
3rd Generation lentiviral vector
|
|
pCCL-PGK-SPdCas9-BFP-DNMT3B |
dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3B catalytic domain
3rd Generation lentiviral vector
|
|
pCCL-PGK-SPdCas9-BFP-EGFP |
dSpCas9-BFP-EGFP (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and EGFP
3rd Generation lentiviral vector
|
|
pLV hUbC-dCas9 KRAB-T2A-GFP |
humanized dead Cas9 KRAB T2A GFP (Other) |
|
|
Gersbach
|
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
|
Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP
FUGW
|
|
pEF_dCas9 |
dCas9 (Other) |
human EF1[alpha] |
|
Rinn
|
Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.
Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
|
Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
pNEB193
Tag
/ Fusion Protein- 3xFLAG (C terminal on insert)
|
|
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A) |
dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3A catalytically inactive domain
3rd Generation lentiviral vector
|
|
pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A) |
dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A (Other) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to BFP and the human DNMT3B catalytically inactive domain
3rd Generation lentiviral vector
|
|
pLenti-EF1a-SPdCas9-EGFP-2A-Blast |
dSpCas9-EGFP (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to EGFP
3rd generation lentiviral vector
|
|
pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast |
dSpCas9-DNMT3A catalytic domain (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to human DNMT3A catalytic domain
3rd generation lentiviral vector
|
|
pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast |
dSpCas9-DNMT3B catalytic domain (Synthetic) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to human DNMT3B catalytic domain
3rd generation lentiviral vector
|
|
pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast |
dSpCas9-DNMT3A(E752A) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to the human DNMT3A catalytically inactive domain
3rd generation lentiviral vector
|
|
pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast |
dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A (Other) |
|
|
Luo
|
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
|
dCas9 fused to the human DNMT3B catalytically inactive domain
3rd Generation lentiviral vector
|
|
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro |
humanized dCas9-KRAB T2A Puro (Other), sgRNA |
|
Puromycin |
Gersbach
|
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
|
Express sgRNA and dCas9-KRAB from lentiviral vector
FUGW
|
|
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP |
humanized dCas9-KRAB T2A GFP (Other), sgRNA |
|
|
Gersbach
|
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
|
Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector
FUGW
|
|
pCR1003 |
T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A) |
|
|
Corn
|
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
|
Express Streptococcus pyogenes dCas9 (D10A, H840A)
SPynCas9
|
|
pCR1056 |
T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS |
|
|
Corn
|
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
|
Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
SPynCas9
|
|
pAC1445-pmax-dCas9 |
dCas9 (Synthetic) |
CAGGS + chim intron |
|
Cheng
|
Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.
Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15.
|
dCas9 expressed in pmax vector
pAC90-pmax-DEST
|
|
pAAVS1-NDi-CRISPRi (Gen1) |
dCas9-KRAB-P2A-mCherry (Synthetic), rtTA (Synthetic) |
TRE3G, CAG |
Neomycin (select with G418) |
Conklin
|
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
pAAVS1
|
|
pAAVS1-NDi-CRISPRi (Gen2) |
dCas9-KRAB (Synthetic), rtTA (Synthetic) |
TRE3G, CAG |
Neomycin (select with G418) |
Conklin
|
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
pAAVS1
|
|
pAAVS1-NC-CRISPRi (Gen3) |
dCas9-KRAB (Synthetic) |
CAG |
Neomycin (select with G418) |
Conklin
|
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
pAAVS1
|
|
pHAGE-TO-dCas9 |
Sp dCas9 (Synthetic) |
CMV-TO |
|
Pederson
|
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
dCas9
pHAGE-DEST
|
|
WN10150 |
dead (908A) AsCpf1 |
|
Neomycin (select with G418) |
Welker
|
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses dead (908A) AsCpf1
pcDNA3.1
|
|
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro |
sgRNA and dCas9 from pX330 (Other) |
U6 for sgRNA and CBh for dCas9 |
Puromycin |
Kimura
|
Dr. Sekita CRISPR/Cas9
(unpublished)
|
Lentivirus vector to express guideRNA and dCas9 with puro resistant gene
CSII
|
|
pLV-dCas9-KRAB-PGK-HygR |
humanized dead Cas9 KRAB (Other), aminoglycoside phosphotransferase from E. coli (Other) |
Human Ubiquitin C Promoter, mouse phosphoglycerate kinase 1 promoter |
Hygromycin |
Gersbach
|
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
|
Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
FUGW
|
|
pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9 |
ABI-tagBFP-Sp dCas9 (Arabidopsis thaliana), PYL1-KRAB (Arabidopsis thaliana) |
PGK, CAG |
Puromycin |
Qi
|
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses ABA-inducible KRAB-Sp dCas9
PB
|
|
pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9 |
GAI-tagBFP-Sp dCas9 (Arabidopsis thaliana), GID1-KRAB (Arabidopsis thaliana) |
PGK, CAG |
Puromycin |
Qi
|
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses GA-inducible KRAB-Sp dCas9
PB
|
|
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9 |
KRAB-tagBFP-Sp dCas9 (Synthetic), Puro |
PGK, CAG |
Puromycin |
Qi
|
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.
Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
|
Expresses direct fusion KRAB-Sp dCas9
PB
|
|
pGH125_dCas9-Blast |
dCas9 and Blasticidin resistance |
|
Blasticidin |
Bassik
|
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for dCas9 with Blasticidin selectable marker
Addgene #61425
|
|
TRE-KRAB-dCas9-IRES-BFP |
KRAB-dCas9-IRES-BFP (Synthetic) |
TRE3G |
BFP |
Lander
|
Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Science. 2016 Sep 29. pii: aag2445.
|
Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP
pHR
|
|
TRE-KRAB-dCas9-IRES-GFP |
KRAB-dCas9-IRES-GFP (Synthetic) |
TRE3G |
GFP |
Lander
|
Systematic mapping of functional enhancer-promoter connections with CRISPR interference.
Science. 2016 Sep 29. pii: aag2445.
|
2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP
pHR
|
|
pMH0001 |
dCas9 (Other) |
SFFV |
BFP fluorescence |
Weissman
|
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.
Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 10.1016/j.cell.2016.11.048.
|
UCOE-SFFV-dCas9-BFP-KRAB
pHR-SFFV-dCas9-BFP-KRAB
|
|
pTE4565 |
hMbCpf1(D986A) |
CMV |
Neomycin (select with G418) |
Welker
|
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses inactive/dead, humanized MbCpf1 nuclease
pcDNA3.1
|
|
pTE4889 |
Fn crRNA, hFnCpf1(D917A) |
human U6, CMV |
Neomycin (select with G418) |
Welker
|
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease
pcDNA3.1
|
|
Lenti-dCas9-KRAB-blast |
dCas9 (Synthetic) |
|
Blasticidin |
Hon
|
Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells.
Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13.
|
Plasmid expression dCas9 protein in fusion with KRAB domain
plenti
|
|
pLKO5d.SFFV.dCas9-KRAB.P2A.BSD |
dCas9 (Other), na, SFFV (Homo sapiens), P2A-BSD (Synthetic) |
|
Blasticidin |
Heckl
|
The non-coding RNA landscape of human hematopoiesis and leukemia.
Nat Commun. 2017 Aug 9;8(1):218. doi: 10.1038/s41467-017-00212-4.
|
CRISPR interference
pLKO5d
|
|
pX330-HA-dSpCas9 |
dead/inactive SpCas9 (Other) |
Cbh |
|
Welker
|
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
|
pX330-Flag-dSpCas9 |
dead/inactive SpCas9 with FLAG tag (Other) |
Cbh |
|
Welker
|
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
|
pX330-Flag-deSpCas9 |
dead/inactive eSpCas9 with FLAG tag (Other) |
Cbh |
|
Welker
|
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
|
pX330-Flag-dSpCas9-HF1 |
dead/inactive SpCas9-HF1 with FLAG tag (Other) |
Cbh |
|
Welker
|
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
|
pX330-Flag-dHeFSpCas9 |
dead/inactive HeFSpCas9 with FLAG tag (Other) |
Cbh |
|
Welker
|
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
|
pLX_311-KRAB-dCas9 |
dCas9 (Other) |
EF1a |
Blasticidin |
Hahn
|
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression.
Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403.
|
for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121
pXPR
Tag
/ Fusion Protein- KRAB (N terminal on insert)
|
|
pXPR_123 |
dCas9 (Other) |
EF1a |
Blasticidin |
Root
|
Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18.
|
for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.
pXPR
Tag
/ Fusion Protein- p300 (C terminal on insert)
|
|
lenti-EF1a-dCas9-KRAB-Puro |
dCas9-KRAB-T2A-Puro (Other) |
EF-1a |
Puromycin, Zeocin |
Brennand
|
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.
Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27.
|
3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)
pLenti
|
|
pHR-EF1a-dCas9-HA-BFP-KRAB-NLS |
dCas9-HA-BFP-KRAB-NLS (Homo sapiens) |
EF1a |
TagBFP |
Corn
|
Corn Lab Cas9 plasmids
(unpublished)
|
Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
pHR
Tag
/ Fusion Protein- HA-TagBFP-KRAB-NLS (C terminal on insert)
|
|
pCXLE-dCas9GFP-shP53 |
dCas9GFP-shP53 (Other) |
CAG |
|
Otonkoski
|
Human pluripotent reprogramming with CRISPR activators.
Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
|
EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.
pCXLE
|
|
PB-tight-DDdCas9GFP-IRES-Neo |
DDdCas9GFP-IRES-Neo (Other) |
TRE-tight |
Neomycin (select with G418) |
Otonkoski
|
Human pluripotent reprogramming with CRISPR activators.
Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
|
PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.
PB-tight
|
|
pAW91.dCas9 |
dCas9 |
|
Blasticidin |
Young
|
YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
|
Expresses dCas9
dcas9-vp64
|
|
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA |
dLbCpf1 (D832A) (Other) |
CAG |
|
Joung
|
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
|
Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
pCAG-GFP
|
|
AAV CMV-dSaCas9-KRAB-bGHpA |
dead S. aureus Cas9 KRAB (Other) |
CMV |
|
Gersbach
|
RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
|
AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor
pAAV
|
|
pLV hUbC-dSaCas9-KRAB-T2A-PuroR |
dead S. aureus Cas9 KRAB T2A PuroR (Other) |
hUbC |
Puromycin |
Gersbach
|
RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors.
Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4.
|
Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB
FUGW
|
|
SID4x-dCas9-KRAB |
SID4x (Homo sapiens), KRAB (Homo sapiens) |
|
Neomycin (select with G418) |
Gertz
|
Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
|
Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
pAC95-pmax (Addgene: 48227)
|
|
AAV-nEF-dCas9 |
nEF-dCas9 |
nEF |
|
Belmonte
|
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.
|
Expresses SpdCas9 from the nEF promoter in AAV backbone
AAV
|
|
pdCas9-NED |
|
CMV |
|
Kiss
|
Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing.
Methods Mol Biol. 2018;1767:395-415. doi: 10.1007/978-1-4939-7774-1_22.
|
Mammalian vector for expressing effector domains fused to dCas9.
pMLM3705 (Addgene plasmid #47754).
Tag
/ Fusion Protein- dCas9, SV40 NLS, 3xFLAG (N terminal on backbone)
|
|
pHAGE EF1α dCas9-NED |
|
EF-1α |
Puromycin |
Kiss
|
Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing.
Methods Mol Biol. 2018;1767:395-415. doi: 10.1007/978-1-4939-7774-1_22.
|
Lentiviral vector for expressing effector domains fused to dCas9.
pHAGE EF1α dCas9-VP64 (Addgene plasmid #50918)
Tag
/ Fusion Protein- 3xHA (N terminal on backbone)
|
|
dCas9-KRAB |
dCas9-KRAB (Synthetic) |
CMV |
|
Church
|
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
Conventional dCas9 repressor-dCas9-KRAB
pcDNA3.3_TOPO
|
|
dCas9-KRAB-MeCP2 |
dCas9-KRAB-MeCP2 (Synthetic) |
CMV |
|
Church
|
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
Improved dCas9 repressor-dCas9-KRAB-MeCP2
pcDNA3.3_TOPO
|
|
pB-CAGGS-dCas9-KRAB |
dCas9-KRAB (Synthetic) |
CAGGS |
Blasticidin |
Church
|
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9-KRAB
pB-CAGGS
|
|
pB-CAGGS-dCas9 |
dCas9 (Synthetic) |
CAGGS |
Blasticidin |
Church
|
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9
pB-CAGGS
|
|
pB-CAGGS-dCas9-KRAB-MeCP2 |
dCas9-KRAB-MeCP2 (Synthetic) |
CAGGS |
Blasticidin |
Church
|
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
|
PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
pB-CAGGS
|
|
pCR1063 |
dCas9-KRAB (Homo sapiens) |
|
|
Corn
|
CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.
Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27.
|
Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg
pLentiXI
Tag
/ Fusion Protein- mCherry (C terminal on insert)
|
|
pLL7 lenti-dcas9-3xflag-blast |
dCas9(D10A, N863A)-T2A-Blast (Synthetic) |
EF1a |
Blasticidin |
Meng
|
Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
lenti dCAS-VP64_Blast
|
|
KRAB-dCas9 |
KRAB (Homo sapiens) |
CMV |
Neomycin (select with G418) |
Segal
|
dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression.
Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578.
|
KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
pcDNA3.3-TOPO
ZNF10 KOX1
|
|
lentiCRISPR v2-dCas9 |
dCas9 (Synthetic), Porumycin resistance (Other) |
EFS-NS, EFS-NS |
Puromycin |
Gilmore
|
lentiviral expression plasmid for gRNA, puromycin, and dCas9
(unpublished)
|
Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
Custom
|
|
pXPR_118 |
dCas9 (Other) |
|
Hygromycin |
Doench
|
Doench CRISPR plasmids
(unpublished)
|
Lentiviral vector expressing dCas9
pXPR_118
|
|
pJEP302-pAAV-CMV-dSaCas9-KRAB-pA |
de-catalyzed SaCas9 (Synthetic) |
|
|
Ploski
|
The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase.
Front. Mol. Neurosci. (2018) 13
|
A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.
AAV
|
|
pJEP305-pAAV-EFS-dSaCas9-KRAB-pA |
de-catalyzed SaCas9 (Synthetic) |
|
|
Ploski
|
The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase.
Front. Mol. Neurosci. (2018) 13
|
A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.
AAV
|
|
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA |
de-catalyzed SaCas9 (Synthetic) |
|
|
Ploski
|
The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase.
Front. Mol. Neurosci. (2018) 13
|
An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.
AAV
|
|
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR |
KRAB-dCas9-P2A-BlastR (Homo sapiens) |
EF1a core and U6 |
Blasticidin |
Ferrer
|
Ferrer Lab Plasmids
(unpublished)
|
Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
lentiCRISPRv2
Tag
/ Fusion Protein- HA (C terminal on insert)
|
|
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR |
KRAB-dCas9-P2A-BlastR (Homo sapiens) |
hPGK and U6 |
Blasticidin |
Ferrer
|
Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes.
Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 10.1038/s41588-019-0457-0.
|
Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
lentiCRISPRv2
Tag
/ Fusion Protein- HA (C terminal on insert)
|
|
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP |
KRAB-dCas9-P2A-EGFP (Homo sapiens) |
EF1a core and U6 |
|
Ferrer
|
Ferrer Lab Plasmids
(unpublished)
|
Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
lentiCRISPRv2
Tag
/ Fusion Protein- HA (C terminal on insert)
|
|
pLenti_dNmeCas9 |
dNmeCas9 (Other) |
|
Puromycin |
Sontheimer
|
NmeCas9 is an intrinsically high-fidelity genome editing platform
(unpublished)
|
dNmeCas9 with no sgRNA cassette lenti construct
pLenti
|
|
pHR-PGK-ABI-dCas9-P2A-mcherry |
dCas9 (Other) |
PGK |
mcherry fluorescence |
Qi
|
CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization.
Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
|
expression of AB1-fused-dcas9 plasmids
pHR
|
|
pHAGE-TO-dCas9-P2A-HSA |
dCas9-P2A-HSA (Synthetic) |
CMV-TO |
|
Pederson
|
CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pHAGE-TO
|
|
lenti_dCas9-KRAB-MeCP2 |
dCas9-KRAB-MeCP2 (Synthetic) |
|
Blasticidin |
Califano
|
Validation of VIPER-inferred master regulators by pooled CRISPR screening
(unpublished)
|
Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
pMH0001
|
|
PB-TRE-dCas9-KRAB-MeCP2 |
dCas9-KRAB-MeCP2 (Synthetic) |
|
Hygromycin |
Califano
|
Validation of VIPER-inferred master regulators by pooled CRISPR screening
(unpublished)
|
SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression
PB-TRE
|
|
MTK3b_030 |
NLS::dCAS9::NLS |
|
|
El-Samad
|
A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells.
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
|
Encodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system
MTK0_027
|
|
PB_tre_dCas9_KRAB |
dCas9 fusion with KRAB, Hygromycin resistance |
TRE, EF1 alpha |
Hygromycin |
Calabrese
|
A piggyBac-based toolkit for inducible genome editing in mammalian cells.
RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.
|
PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion
piggyBac cargo vector
|
|
pMTL3 |
AAVS1-CAG-KRAB-dCas9-BFP-KRAB (Synthetic) |
CAG |
Puromycin |
Kampmann
|
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
|
constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
pAAVS1
|
|
pMTL5 |
AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB (Synthetic) |
CAG |
Puromycin |
Kampmann
|
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
|
DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
pAAVS1
|
|
pC13N-dCas9-BFP-KRAB |
CLYBL-CAG-dCas9-NLS-BFP-KRAB (Synthetic) |
CAG |
Neomycin (select with G418) ; BFP |
Kampmann
|
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
|
constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)
pC13N-iRMCE
|
|
pRT029 |
CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR (Synthetic) |
CAG |
BFP |
Kampmann
|
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
|
double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
pC13N
|
|
pAT1089_PB-EF1a-Blast_2A_rtTA3-SV40pA_pCW-dCas9 |
HA-dCas9 (Other) |
TET, TET_ON (Dox inducible) |
Blasticidin |
Cheng
|
Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways.
Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
|
PiggyBac vector for establishement of doxycycline-inducible Sp dCas9 expression cell lines
pAT1502
|
|
pMH0006 |
dCas9-BFP-KRAB (Other) |
EF1A |
tagBFP |
Weissman
|
Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation.
J Biol Chem. 2019 Oct 2. pii: RA119.009432. doi: 10.1074/jbc.RA119.009432.
|
Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.
pHR-SFFV-dCas9-BFP-KRAB
|
|
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1 |
BirA (Other), dCas9 (Other) |
EF1a, EF1a |
zsGreen1 |
Xu
|
Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9.
Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
|
CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1
pLVX-EF1a-zsGreen1
|
|
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 |
dCas9 (Other) |
EF1a |
zsGreen1 |
Xu
|
Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9.
Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
|
CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1
pLVX-EF1a-zsGreen1
|
|
CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1 |
dCas9 (Other) |
EF1a |
zsGreen1 |
Xu
|
Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9.
Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w.
|
CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1
pLVX-EF1a-zsGreen1
|
|
pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE |
KRAB-dSpCas9 (Other) |
|
Puromycin |
Sanjana
|
High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
|
pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE |
KRAB-dSpCas9-NG (Other) |
|
Puromycin |
Sanjana
|
High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
|
pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE |
KRAB-dxCas9 3.7 (Other) |
|
Puromycin |
Sanjana
|
High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
|
pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE |
KRAB-dxCas9-NG (Other) |
|
Puromycin |
Sanjana
|
High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
|
XClone-Cas9-Cronus-T2A-BFP |
Cas9 (Other) |
|
Blasticidin |
Johnson
|
Tunable and temporal expression control of Cas9
(unpublished)
|
Tunable and temporal expression control of Cas9
XLone-GFP
|