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Functional disruption of the prion protein gene in cloned goats
- Guohua Yu1,2, Jianquan Chen2,3, Huiqing Yu2,3, Siguo Liu2,3, Juan Chen2,3, Xujun Xu2,3, Hongying Sha2,3, Xufeng Zhang2,3, Guoxiang Wu2,3, Shaofu Xu2,3, Guoxiang Cheng2,3
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View Affiliations Hide AffiliationsAffiliations: 1 1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China 2 2Shanghai Transgenic Research Center, 88 Cai-Lun Road, Shanghai 201203, China 3 3Shanghai Genon Bio-Engineering Co. Ltd, 88 Cai-Lun Road, Shanghai 201203, ChinaCorrespondenceGuoxiang Cheng [email protected]
- First Published: 01 April 2006 https://doi.org/10.1099/vir.0.81384-0
Abstract
The cellular prion protein (PrPC), a membrane glycoprotein anchored to the outer surface of neurons, lymphocytes and other cells, is associated directly with the pathogenesis of the transmissible spongiform encephalopathies (TSEs) occurring mainly in humans, cattle, sheep and goats. Although mice lacking PrPC develop and reproduce normally and are resistant to scrapie infection, large animals lacking PrPC, especially those species in which TSE occurs naturally, are currently not available. Here, five live PRNP +/− goats cloned by gene targeting are reported. Detailed RNA-transcription and protein-expression analysis of one PRNP +/− goat showed that one allele of the caprine PRNP gene had been disrupted functionally. No gross abnormal development or behaviour could be seen in these PRNP +/− goats up to at least 3 months of age. These heterozygous PRNP +/− goats are ready to be used in producing homozygous PRNP −/− goats in which no PrPC should be expressed.
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