Regeneration of genome-edited events by delivering CRISPR/Cas9 construct through Agrobacterium-mediated transformation of cell suspension of Gonja Manjaya. a Schematic representation of T-DNA region of binary vector pMR215-BSV1 showing the positions of three gRNAs. LB, left border; RB, right border; PAM, protospacer adjacent motif. Sequences of the gRNAs are also indicated. b and c Germination of Agro-infected embryos in selective medium containing kanamycin. d Well-rooted plantlets in proliferation medium. e Genome-edited Gonja Manjaya plants in glasshouse. f Non-edited wild type control Gonja Manjaya plants in glasshouse