Sequence analysis of genome-edited plants of Gonja Manjaya to detect mutations in S1 and S2 target sites. Nucleotides in brown represent the target sites and blue nucleotides indicate protospacer adjacent motif (PAM). Dotted lines (…) represent deletions, nucleotides in red indicate insertion, and green nucleotides indicate replacement. BSOLV, sequence of BSV strain Obino l’Ewai; WT, sequences same as wild type non-edited control Gonja Manjaya; 1–100, 20 independent transgenic events. The mutations in S1, S2, and S1/S2, number of indels as deletions (−), insertions (+) or replacements (r) and percentage of clones of transgenic event showing same mutations are indicated on the right side panel. The PCR products amplified using primer P1 (see Fig. 1b) were purified, cloned, and 10 individual clones per event were analyzed for mutations at target sites S1 and S2 by Sanger sequencing