Table 1 Number of potential off-target sites for mutations in A and B genomes of plantain for the three CRISPR targets in eBSOLV integrated in Gonja Manjaya (AAB)
gRNA sequence from eBSOLV + PAM | Number of potential off-targets | |
---|---|---|
A genome | B genome | |
TTGAGCAAAGAAGACGTTGAGG | 11 | 9 |
GCTGGAACAACTGGTGACTGGG | 13 | 6 |
ATGGAGTTCATATGATCATAGG | 34 | 25 |