Table 2 Selected potential off-target sites used for sequence analyzing of genome-edited events of Gonja Manjaya (AAB)

From: CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

gRNA sequence from eBSOLV + PAM A genome B genome
  Potential off-target site (Gene ID) Identity (%) Sequence alignment Potential off-target site (Gene ID) Identity (%) Sequence alignment
TTGAGCAAAGAAGACGTTGAGG Ma05_t06600.1 77.27 GAGCAAAGAAGACGTTGA
| | |  | | |  |  | |  | | |  | | | | |
GAGCAAAGAAGAAGTTGA 		ITC1587_Bchr5_T12273 77.27 GAGCAAAGAAGACGTTGA
| | |  | |  | |  | |  |  | | |  | | | |GAGCAAAGAAGAAGTTGA
GCTGGAACAACTGGTGACTGGG Ma05_t31620.1 95.65 TGCTGGAACAACTGGTGACTGGG
 | |  | | | |  | |  | | | | | |  |  | | | | |
TGCTGAAAAATCTGGTGACTGGG 		ITC1587_Bchr4_T08704 63.64 GCTGGAACAACTGGT
| | | |  | | |  | | |  | | |  | |
GCTGGAACAACTGGT
Ma01_t10610.1 86.36 CTGGAACAACTGGTGACTGG
 |  | |  | |  |  |  |  |  |  |  |  |  |  |  |  | |
CTGGTCCAACTGGTGACTGG 		     
ATGGAGTTCATATGATCATAGG Ma08_t27880.1 86.36 ATGGAGTTCATATGATCATA
 |  |  |  |  |   |  | |  | | | |  | | | | | |
ATGGAGACCATATGATCATA 		ITC1587_Bchr1_T00896 86.36 TGGAGTTCATATGATCATAG
 | |  | |  | | | | | | |  | |  |   | |  |  |
TGGAGTTCATATGTTTATAG
  1. Seven putative off-target loci containing high sequence similarity to the gRNAs or/and PAM sequence (NGG) were selected for sequencing of nine genome-edited events. The PAM sequences are shown in italics
Back to article page