A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants
- PMID: 28211909
- DOI: 10.1038/nplants.2017.18
A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants
Erratum in
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A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants.Nat Plants. 2017 Jun 19;3:17103. doi: 10.1038/nplants.2017.103. Nat Plants. 2017. PMID: 28628131
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice T0 transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR-Cpf1 for editing plant genomes and modulating the plant transcriptome.
Comment in
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Genome editing: The efficient tool CRISPR-Cpf1.Nat Plants. 2017 Mar 3;3:17028. doi: 10.1038/nplants.2017.28. Nat Plants. 2017. PMID: 28260792 No abstract available.
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