CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination
- PMID: 30673818
- DOI: 10.1007/s00299-019-02379-0
CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination
Abstract
We summarize recent progress of CRISPR/Cas9-mediated gene targeting in plants, provide recommendations for designing gene-targeting vectors and highlight the potential of new technologies applicable to plants. Gene targeting (GT) is a tool of urgent need for plant biotechnology and breeding. It is based on homologous recombination that is able to precisely introduce desired modifications within a target locus. However, its low efficiency in higher plants is a major barrier for its application. Using site-specific nucleases, such as the recent CRISPR/Cas system, GT has become applicable in plants, via the induction of double-strand breaks, although still at a too low efficiency for most practical applications in crops. Recently, a variety of promising new improvements regarding the efficiency of GT has been reported by several groups. It turns out that GT can be enhanced by cell-type-specific expression of Cas nucleases, by the use of self-amplified GT-vector DNA or by manipulation of DNA repair pathways. Here, we highlight the most recent progress of GT in plants. Moreover, we provide suggestions on how to use the technology efficiently, based on the mechanisms of DNA repair, and highlight several of the newest GT strategies in yeast or mammals that are potentially applicable to plants. Using the full potential of GT technology will definitely help us pave the way in enhancing crop yields and food safety for an ecologically friendly agriculture.
Keywords: Cas12; Cas9; Double-strand break; Gene targeting; Homologous recombination.
Similar articles
-
The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.Plant J. 2014 Dec;80(6):1139-50. doi: 10.1111/tpj.12704. Epub 2014 Nov 11. Plant J. 2014. PMID: 25327456
-
CRISPR/Cas-Mediated In Planta Gene Targeting.Methods Mol Biol. 2017;1610:3-11. doi: 10.1007/978-1-4939-7003-2_1. Methods Mol Biol. 2017. PMID: 28439853
-
TALEN- and CRISPR-enhanced DNA homologous recombination for gene editing in zebrafish.Methods Cell Biol. 2016;135:107-20. doi: 10.1016/bs.mcb.2016.03.005. Epub 2016 Apr 7. Methods Cell Biol. 2016. PMID: 27443922
-
Homology-based double-strand break-induced genome engineering in plants.Plant Cell Rep. 2016 Jul;35(7):1429-38. doi: 10.1007/s00299-016-1981-3. Epub 2016 Apr 15. Plant Cell Rep. 2016. PMID: 27084537 Review.
-
Precise Genome Modification via Sequence-Specific Nucleases-Mediated Gene Targeting for Crop Improvement.Front Plant Sci. 2016 Dec 20;7:1928. doi: 10.3389/fpls.2016.01928. eCollection 2016. Front Plant Sci. 2016. PMID: 28066481 Free PMC article. Review.
Cited by 9 articles
-
Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants.Genes (Basel). 2020 Apr 24;11(4):466. doi: 10.3390/genes11040466. Genes (Basel). 2020. PMID: 32344599 Free PMC article. Review.
-
High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice.Sci Rep. 2019 Dec 27;9(1):19902. doi: 10.1038/s41598-019-55681-y. Sci Rep. 2019. PMID: 31882637 Free PMC article.
-
DNA Helicases as Safekeepers of Genome Stability in Plants.Genes (Basel). 2019 Dec 10;10(12):1028. doi: 10.3390/genes10121028. Genes (Basel). 2019. PMID: 31835565 Free PMC article. Review.
-
Systems genomics approaches provide new insights into Arabidopsis thaliana root growth regulation under combinatorial mineral nutrient limitation.PLoS Genet. 2019 Nov 6;15(11):e1008392. doi: 10.1371/journal.pgen.1008392. eCollection 2019 Nov. PLoS Genet. 2019. PMID: 31693663 Free PMC article.
-
Gene drives in plants: opportunities and challenges for weed control and engineered resilience.Proc Biol Sci. 2019 Sep 25;286(1911):20191515. doi: 10.1098/rspb.2019.1515. Epub 2019 Sep 25. Proc Biol Sci. 2019. PMID: 31551052
References
Publication types
MeSH terms
Grant support
LinkOut - more resources
-
Full Text Sources
-
Miscellaneous