Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion
- PMID: 18303117
- PMCID: PMC2265136
- DOI: 10.1073/pnas.0707881105
Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion
Abstract
Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other plants that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such as mutagenesis). What is beyond the phenotype of these improved plants? Should mutagenized plants be treated differently from transgenics? We have evaluated the extent of transcriptome modification occurring during rice improvement through transgenesis versus mutation breeding. We used oligonucleotide microarrays to analyze gene expression in four different pools of four types of rice plants and respective controls: (i) a gamma-irradiated stable mutant, (ii) the M1 generation of a 100-Gy gamma-irradiated plant, (iii) a stable transgenic plant obtained for production of an anticancer antibody, and (iv) the T1 generation of a transgenic plant produced aiming for abiotic stress improvement, and all of the unmodified original genotypes as controls. We found that the improvement of a plant variety through the acquisition of a new desired trait, using either mutagenesis or transgenesis, may cause stress and thus lead to an altered expression of untargeted genes. In all of the cases studied, the observed alteration was more extensive in mutagenized than in transgenic plants. We propose that the safety assessment of improved plant varieties should be carried out on a case-by-case basis and not simply restricted to foods obtained through genetic engineering.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Similar articles
-
Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials.Food Chem Toxicol. 2008 Mar;46 Suppl 1:S2-70. doi: 10.1016/j.fct.2008.02.008. Epub 2008 Feb 13. Food Chem Toxicol. 2008. PMID: 18328408 Review.
-
Only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene.Plant Biotechnol J. 2011 Aug;9(6):693-702. doi: 10.1111/j.1467-7652.2010.00572.x. Epub 2010 Oct 29. Plant Biotechnol J. 2011. PMID: 21040388
-
Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3.BMC Genomics. 2010 Jan 28;11:69. doi: 10.1186/1471-2164-11-69. BMC Genomics. 2010. PMID: 20105335 Free PMC article.
-
Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes.Plant Biotechnol J. 2009 Aug;7(6):512-26. doi: 10.1111/j.1467-7652.2009.00420.x. Epub 2009 May 21. Plant Biotechnol J. 2009. PMID: 19490479
-
Comparative safety assessment of plant-derived foods.Regul Toxicol Pharmacol. 2008 Feb;50(1):98-113. doi: 10.1016/j.yrtph.2007.09.007. Epub 2007 Sep 29. Regul Toxicol Pharmacol. 2008. PMID: 17983697 Review.
Cited by 36 articles
-
Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic Res. 2020 Jun;29(3):321-337. doi: 10.1007/s11248-020-00203-0. Epub 2020 Apr 30. Transgenic Res. 2020. PMID: 32356192 Free PMC article.
-
Familiarity in the Context of Risk Assessment of Transgenic Crops: Focus on Some Countries in the Americas.Front Bioeng Biotechnol. 2020 Jan 28;7:463. doi: 10.3389/fbioe.2019.00463. eCollection 2019. Front Bioeng Biotechnol. 2020. PMID: 32047744 Free PMC article.
-
Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?Front Plant Sci. 2019 Dec 11;10:1592. doi: 10.3389/fpls.2019.01592. eCollection 2019. Front Plant Sci. 2019. PMID: 31921242 Free PMC article. Review.
-
Responses of PYR/PYL/RCAR ABA Receptors to Contrasting stresses, Heat and Cold in Arabidopsis.Plant Signal Behav. 2019;14(12):1670596. doi: 10.1080/15592324.2019.1670596. Epub 2019 Sep 25. Plant Signal Behav. 2019. PMID: 31552801
-
CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement.Sci Rep. 2019 Apr 30;9(1):6729. doi: 10.1038/s41598-019-43141-6. Sci Rep. 2019. PMID: 31040331 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
-
Full Text Sources
-
Other Literature Sources