High-throughput functional genomics using CRISPR-Cas9
- PMID: 25854182
- PMCID: PMC4503232
- DOI: 10.1038/nrg3899
High-throughput functional genomics using CRISPR-Cas9
Abstract
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
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Comment in
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Genetic screens: Combination screens for combination therapies.Nat Rev Genet. 2016 Jun;17(6):313. doi: 10.1038/nrg.2016.52. Epub 2016 Apr 12. Nat Rev Genet. 2016. PMID: 27067263 No abstract available.
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