CAP-C uses multifunctional DNA crosslinkers of defined sizes to increase resolution and signal-to-noise in chromosome conformation capture experiments.

Combining droplet-based ATAC-seq and mitochondrial DNA sequencing reveals clonal variation in human cells and tissues.

A new imaging method enables deep imaging of neural networks.

scVelo reconstructs transient cell states and differentiation pathways from single-cell RNA-sequencing data.

SARS-CoV-2 is reliably detected in nasal swab samples using mass spectrometry and machine learning analysis.

Cell clones in lineage-tracing studies can be isolated for functional experiments.

A system to express neoantigens in tumor cells and mice circumvents central T cell tolerance.

A blocking assay based on the recombinant receptor-binding domain of SARS-CoV-2 spike protein and human angiotensin-converting enzyme 2 receptor provides an alternative to conventional antibody neutralization assays requiring live virus.

New base editors change C to A in bacteria and C to G in mammalian cells.

A new base editor enables the creation of C-to-G base changes in human cells.

The cell types affected by biological perturbations in complex tissues are uncovered by single-cell analysis.

More than 200,000 gut prokaryotic reference genomes and the proteins they encode are collated, providing comprehensive resources for microbiome researchers.

CentroFlye resolves tandem repeats to assemble human centromeres from nanopore reads.

An algorithm to design nonrepetitive genetic parts enables synthetic biologists to reliably engineer stable circuits.

A new safety switch allows for control of cell therapies by dietary uridine.

Improved Cas12a variants and sgRNA design rules enhance genome-wide screens.

Off-the-shelf CAR T cells avoid immune rejection in mouse models of allogeneic cancer cell therapy.

Equipping a protein nanopore with a second constriction improves sequencing of homopolymer DNA stretches.

The activity of adenine or cytosine base editors at specific target nucleotides is predicted computationally.

Water contaminants are detected with freeze-dried, regulated in vitro transcription reactions.

Rice genes are replaced using chemically modified donor DNA and CRISPR targeting.

The rabbit uterus is reconstructed using synthetic scaffolds seeded with cells.

As CRISPR therapies move into clinical testing, David Schaffer and colleagues review a raft of different delivery technologies being road tested to address cargo capacity limitations, maximize potency, minimize off-target effects and avoid immunogenicity.

The most abundant RNA modification in humans, m⁶A, can be installed at specified RNA sequences in cells, enabling functional studies.

A suite of APOBEC–Cas9 complexes for predictable and targeted deletions in plant genomes.

Microscopy datasets are processed orders-of-magnitude faster with improved algorithms and deep learning.

Transient p53 inhibition and induced cell-cycle progression increase clonal engraftment and homology-directed repair in hematopoietic stem cells.

Single-cell analysis of COVID-19 patient samples identifies activated immune pathways that correlate with severe disease.

A growing arsenal of CRISPR-based tools enables increasingly sophisticated genome editing applications.

Detection of structural variants in the human genome is facilitated by a benchmark set of large deletions and insertions.

Gut microbiome composition is remodeled using small peptides.

Mass spectral fragmentations of lipids across 117 lipid subclasses are presented in a lipidome atlas.

The genome-wide activity of Cas9 is measured in unprecedented detail.

Deep-learning models predict the Cas9 variant with optimal activity and specificity for any target sequence.

Base editors that modify both adenosine and cytosine broaden the potential applications of base editing.

Base editors that modify both adenine and cytosine broaden the potential applications of base editing.

A base editor that concurrently modifies both adenine and cytosine broadens the potential applications of base editing.