Fig 2

(A) Geographical locations of proclaimed TR4 incursions in Southeast Asia, Australia, Africa, the Middle East, and the Indian subcontinent. Different colors indicate if and how the genetic diversity of collected isolates was assessed. (B) Limited genetic diversity between multiple Foc TR4 isolates from distinct geographical locations revealed by hierarchical clustering, based on 4,298 DArTseq markers. Countries of origin for each of the TR4 isolates are indicated by different colors. (C) Phylogenetic analysis of selected Foc TR4 isolates (highlighted in bold in panel B) and related F. oxysporum species, based on whole-genome re-sequencing data. Phylogenetic tree analysis was performed using REALPHY [37], applying the PhyML algorithm for tree constructing (Foc II5 reference genome). The F. oxysporum f.sp. lycopersici and the F. oxysporum f. sp. cubense II5 genomes, as well as Foc race 4 and race 1 genomes, are publicly available at GenBank (http://www.ncbi.nlm.nih.gov/genome/genomes/707). Robustness of the grouping was assessed by 500 bootstrap replicates, and thick branches indicate maximum support.