A cryo–electron microscopy structure of the base editor ABE8e in a substrate-bound state elucidates base-editing mechanisms.
Phage-derived CasΦ uses a single active site to process guide RNA and cut DNA for genome editing and nucleic acid detection.
CRISPR-Cas9 gene-edited T cells show safety and long-term engraftment in humans
Identification, characterization, and technological implementation of additional archaea-derived CRISPR-Cas14 systems are described.
CRISPR-Cas12a inhibitors that block gene editing in human cells are identified.
Single-stranded DNase activity upon guide RNA–dependent DNA binding can be harnessed for rapid and specific nucleic acid detection.
The CRISPR integration complex relies on structural features of DNA to achieve site-selective CRISPR array expansion.
A conformational checkpoint of Cas9 enables on-target cleavage while blocking access to the active state at off-target sites.
