Production of hornless dairy cattle from genome-edited cell lines

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Figure 1: Phenotypic and genotypic confirmation of POLLED introgression in Spotigy and Buri.


  1. 1

    Bouie, F. A. The dehorning debate. Farm Animal Welfare News 2 (2002).

  2. 2

    Spurlock, D.M., Stock, M.L. & Coetzee, J.F. J. Dairy Sci. 97, 5265–5274 (2014).

    CAS  Article  Google Scholar 

  3. 3

    Stafford, K.J. & Mellor, D.J. Vet. J. 169, 337–349 (2005).

    CAS  Article  Google Scholar 

  4. 4

    Wilcox, C.S., Schutz, M.M., Rostagno, M.R., Lay, D.C. Jr. & Eicher, S.D. J. Dairy Sci. 96, 7223–7233 (2013).

    CAS  Article  Google Scholar 

  5. 5

    Swanson, A.F. Wanted: more bulls with no horns. NPR The Salt (3 August, 2015).

    Google Scholar 

  6. 6

    Dorshorst, B. Half of Holstein heifer calves could be polled by 2034. Progressive Dairyman (17 January, 2014).

    Google Scholar 

  7. 7

    Tan, W.S., Carlson, D.F., Walton, M.W., Fahrenkrug, S.C. & Hackett, P.B. Adv. Genet. 80, 37–97 (2012).

    CAS  Article  Google Scholar 

  8. 8

    Medugorac, I. et al. PLoS One 7, e39477 (2012).

    CAS  Article  Google Scholar 

  9. 9

    Rothammer, S. et al. Genetics Selection Evolution 46, 1–5 (2014).

    Article  Google Scholar 

  10. 10

    Tan, W. et al. Proc. Natl. Acad. Sci. USA 110, 16526–16531 (2013).

    CAS  Article  Google Scholar 

  11. 11

    Ross, P.J. & Cibelli, J.B. Methods Mol. Biol. 636, 155–177 (2010).

    Article  Google Scholar 

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Correspondence to Scott C Fahrenkrug.

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Competing interests

D.F.C., C.A.L., E.S.K., T.S.S. and S.C.F. are employees of Recombinetics; D.F.C., C.A.L., E.S.K., M.W., T.S.S. and S.C.F. are shareholders in Recombinetics.

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Carlson, D., Lancto, C., Zang, B. et al. Production of hornless dairy cattle from genome-edited cell lines. Nat Biotechnol 34, 479–481 (2016).

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