Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CRISPR Plasmids: Plants

Browse CRISPR Plasmids

By Function
Genome Editing
Transcriptional Regulation
RNA Targeting
Other Applications
Screen
gRNAs
By Species

CRISPR Resources

The following CRISPR plasmids have been designed for use in plants.

Cut

Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ.

Plasmid Gene/Insert Selectable Marker PI Publication Hidden Extra Search Info
pK7WGF2::hCas9 hCas9 (Synthetic) Kanamycin Kamoun Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655. Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue pK7WGF2
pICH41308::hCas9 hCas9 (Synthetic) Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Level 0 hCas9 module pICH41308
pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST 35Sp::hCas9 (Synthetic) Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Level 1 hCas9 module pICH47742
pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1 sgRNA_PDS2-Cas9-sgRNA_PDS1 (Synthetic) Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Level 2 construct pAGM4723
pBUN411 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
pHSN401 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
pRGE31 Yang RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter pUGW11
pRGEB31 Hygromycin Yang RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation. pCAMBIA1300
HBT-pcoCas9 pcoCas9 (Synthetic) Sheen Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. transient expression of pcoCas9 gene in plant cells HBT-FLAG
pFGC-pcoCas9 35SPPDK:pcoCas9:NOS cassette (Synthetic) Basta Sheen Sheen lab CRISPR plasmids (unpublished) A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA pFGC-RCS
pJIT163-2NLSCas9 Cas9 (Other) Gao Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. Expression of rice codon-optimized Cas9 in plant cells pJIT163
p201N Cas9 Cas9 (Synthetic), nptII (Other) G418, kanamycin Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
p201H Cas9 Cas9 (Synthetic), hph (Other) Hygromycin Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
p201B Cas9 Cas9 (Synthetic), BAR (Other) Basta Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
p201G Cas9 Cas9 (Synthetic), sGFP (Synthetic) GFP Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
Cas9 MDC123 Glycine Max codon optimized Cas9 (Other) Basta Stupar CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063. 2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection pMDC123
Cas9 MDC32 Glycine Max codon optimized Cas9 (Other) Hygromycin Stupar CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063. 2x35S promoting Glycing Max codon optimized Cas9 with hygromycin plant selection pMDC32
G10 Cas9 MDC123 Glycine Max codon optimized Cas9 (Other) Basta Stupar CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063. G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection pMDC123
pDe-CAS9 Cas9 (Arabidopsis thaliana) Basta Puchta Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence pPZP221
pCAS9-TPC Cas9 (Arabidopsis thaliana) Basta Puchta Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning pPZP221
pBUE411 gRNA scaffold (Synthetic), zCas9 (Other) Basta Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pCambia
pHSE401 gRNA scaffold (Synthetic), zCas9 (Other) Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pCambia
pKSE401 gRNA scaffold (Synthetic), zCas9 (Other) Neomycin (select with G418) Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance pCambia
pHUE411 gRNA scaffold (Synthetic), zCas9 (Other) Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance pCambia
pBUN421 gRNA scaffold (Synthetic), zCas9 (Other) Basta Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance pGreen-like
pRGEB32 Hygromycin Yang Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation. pRGEB31
pRGE32 Yang Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast pRGE31
pYLCRISPR/Cas9Pubi-H Cas9 (Other) Hygromycin Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection pCAMBIA1300
pYLCRISPR/Cas9Pubi-B Cas9 (Other) Basta Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection pCAMBIA1300
pYLCRISPR/Cas9P35S-H Cas9 (Other) Hygromycin Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection pCAMBIA1300
pYLCRISPR/Cas9P35S-B Cas9 (Other) Basta Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection pCAMBIA1300
pYLCRISPR/Cas9P35S-N Cas9 (Other) Neomycin (select with G418) Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection pCAMBIA1300
pEGB2alpha2 35s:hCas9:tNos (GB0639) hCas9 (Other) Orzaez GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31. Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter pDGB2alpha2
pICSL11056 Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s (Other) Patron Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7. Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants pICH47742 (AddGene #47742)
pICSL11060 Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s (Other) Patron Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7. Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea) pICH47742 (AddGene #47742)
pYPQ150 pcoCas9 (plant codon-optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with pcoCas9 (Plant codon optimized) pNJB91
pYPQ154 AteCas9 (Arabidopsis codon optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with AteCas9 (Arabidopsis codon optimized) pYPQ185-linker2
pYPQ158 hSpCas9 (Human codon optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with hSpCas9 (Human codon optimized) pNJB185-linker6
pYPQ167 Cas9p (Plant codon-optimized; high GC content at 5 prime region) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with Cas9p pYPQ185-linker2
pTC217 Nuclease (Cas9/sgRNA) + Donor + GVR (Other) kanamycin Voytas High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b pLSLR
pTC223 Nuclease (Cas9/sgRNA) + Donor + GVR (Other) kanamycin Voytas High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7 pLSLR
pHEE401 sgRNA scaffold (Synthetic), zCas9 (Other) Hygromycin Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia
pHEE401E gRNA scaffold (Synthetic), zCas9 (Other) Hygromycin Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia
pHEE2E-TRI sgRNA targeting TRY and CPC genes (Synthetic), sgRNA targeting ETC2 gene (Synthetic), zCas9 (Other) Hygromycin Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC pCambia
pMpGE010 Hygromycin Kohchi Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. For CRISPR/Cas9 genome editing in Marchantia polymorpha pMpGWB103
pMpGE011 Chlorsulfuron Kohchi Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. For CRISPR/Cas9 genome editing in Marchantia polymorpha pMpGWB303
pEGB2Ω2_SF-35s:hCas9:tNos (GB1103) hCas9 (Other) Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181. pDGB2omega2
pBAtC Basta Kim A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. Plant expression of Cas9 and gRNA empty backbone; Basta resistance pB2GW7
  • Tag / Fusion Protein
    • NLS-HA (C terminal on insert)
    		•
    pHAtC Hygromycin Kim A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance pH2GW7
    pHDE-35S-Cas9-mCherry Hygromycin ; mCherry fluorescence Zhao An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016. For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants pHDE
    pHDE-35S-Cas9-mCherry-UBQ Hygromycin ; mCherry fluorescence Zhao An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016. For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs pHDE
    pKIR1.1 human-codon-optimized SpCas9 (Other) Hygromycin ; TagRFP in seeds Higashiyama pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter pFAST-R01
    pKI1.1 human-codon-optimized SpCas9 (Other) Higashiyama pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO pENTR/D-TOPO
    pKI1.1R human-codon-optimized SpCas9 (Other) Hygromycin ; TagRFP in seeds Higashiyama pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter. pFAST-R01
    pAGM4723:TpCC_Urease Cas9 (Other), Urease-targeting gRNA 1 (Other), Urease-targeting gRNA 2 (Other) Nourseothricin (Nat) Mock Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana Plant Methods 2016, 12:49 Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs pAGM4723
    pICH47742:FCP:Cas9YFP Cas9 (Other) Mock Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana Plant Methods 2016, 12:49 Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter pICH47742
    pYPQ220 (AsCpf1) AsCpf1 (Other) Qi A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. AsCpf1 Gateway entry plasmid pYPQ167
    pYPQ230 (LbCpf1) LbCpf1 (Other) Qi A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. LbCpf1 Gateway entry plasmid pYPQ167
    pKIR1.0 human-codon-optimized SpCas9 (Other) TagRFP in seeds Higashiyama pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter. pFAST-R01
    pUB-Cas9 Cas9 (Synthetic) Hygromycin Weber An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana. Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017. For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017) pKB65
    [email protected] sgRNA against GLABROUS1 (Synthetic) Hygromycin Weber An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana. Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017. Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9 pUB-Cas9
    pTX168 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants pCAMBIA1300
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pTX171 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants pTX168
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pTX172 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants pTX171
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pTX176 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants pTX171
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pJG80 TaCas9 (Other) Voytas High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9. Plant J. 2016 Dec 10. doi: 10.1111/tpj.13446. Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites unknown
    pMOD_A0108 AtCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP pMOD_A1001
    pMOD_A0502 Csy4-P2A-AtCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP pMOD_A1001
    pMOD_A0508 Csy4-P2A-AtCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP pMOD_A1001
    pMOD_A0901 TREX2-P2A-AtCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP pMOD_A1001
    pMOD_A0908 TREX2-P2A-AtCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP pMOD_A1001
    pMOD_A1110 TaCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP pMOD_A1001
    pMOD_A1111 TaCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP pMOD_A1001
    pMOD_A1510 Csy4-P2A-TaCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP pMOD_A1001
    pMOD_A1511 Csy4-P2A-TaCas9 (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP pMOD_A1001
    pDIRECT_21A Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II pTRANS_210
    pDIRECT_21C Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II pTRANS_210d
    pDIRECT_22A Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II pTRANS_220
    pDIRECT_22C Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II pTRANS_220d
    pDIRECT_23A Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar pTRANS_230
    pDIRECT_23C Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar pTRANS_230d
    pDIRECT_25F Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II pTRANS_250d
    pDIRECT_25H Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II pTRANS_250d
    pDIRECT_26H Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar pTRANS_260d
    pAtCas9_1 AtCas9 (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9_3 AtCas9 (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar pCAMBIA
    pTaCas9_5 TaCas9 (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9_6 TaCas9 (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar pCAMBIA
    pDIRECT_10A 35S:AtCas9 + AtU6:gRNA (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none pTRANS_100
    pKEE401 Neomycin (select with G418) Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance pCambia
    pXEE401B Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance pCambia
    pXEE401BG Chen Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance pCambia
    pSC10 Stupar CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857. pAH595-gRNA 2xplex entry cassette pENTR top
    pSC12 Stupar CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857. pNB184-Cas9 entry cassette pENTR top
    pYLCRISPR/Cas9pUbi-N Cas9 (Synthetic) Liu A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007. Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection pCAMBIA1300
    pJKW0458 Weng pJKW0457 (unpublished) p35S:CAS9 pEarleyGate100
    pJKW0474 Weng pJKW0457 (unpublished) pCLV3:Cas9 pEarleyGate100
    pMpGE006 Atco-Cas9-Pea3ter (Synthetic) Hygromycin Sugano Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. Cas9 expression in M.polymorpha pMpGWB103
    pYPQ239 FnCpf1 (Other) Qi Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. FnCpf1 Gateway entry plasmid pYPQ167
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    pYPQ230-RR LbCpf1-RR (LbCpf1 with G548R and K611R mutations) (Other) Qi Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. LbCpf1 Gateway entry plasmid with G548R and K611R mutations pYPQ167
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    pYPQ230-RVR LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations) (Other) Qi Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations pYPQ167
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    pYPQ239-RR FnCpf1-RR (FnCpf1 with N623R and K687R mutations) (Other) Qi Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. FnCpf1 Gateway entry plasmid with N623R and K687R mutations pYPQ167
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    pYPQ239-RVR FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations) (Other) Qi Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations pYPQ167
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    		•
    pORFE0001 AtCAS9 (Other) Chevalier Chevalier Lab plasmids (unpublished) AtCas9 module CDS1 pICH41308
    pORFE1001 AtCAS9 (Other) Chevalier Chevalier Lab plasmids (unpublished) Overexpression of the AtCAS9 in plant under 2x35S promoter pICH47742
    pMH-Cas9-gate Cas9 (Other) Hygromycin Bezanilla Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants. pGEM-T easy
    pMK-Cas9-gate Cas9 (Other) Neomycin (select with G418) Bezanilla Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants. pGEM-T easy
    pZeo-Cas9-gate Cas9 (Other) Zeocin Bezanilla Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants. pGEM-T easy
    BCJJ339 BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI (Arabidopsis thaliana) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 pICH47742
    BCJJ340 BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 UBI10:Cas9:E9 Golden Gate Level 1 Position 2 pICH47742
    BCJJ344 BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
    BCJJ345 BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
    BCJJ358 BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
    pICSL90016 BsaI:AATG:Cas9_2:GCTT:BsaI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 Cas9_2 Golden Gate Level 0 pICH41308
    BCJJ125 BsaI:AATG:Cas9_3(intron):GCTT:BsaI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 Cas9_3 (with intron) Golden Gate Level 0 pICH41308
    BCJJ180 BsaI:AATG:Cas9_4:GCTT:BsaI (Synthetic) Jones Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952 Cas9_4 Golden Gate Level 0 pICH41308
    pICSL90005 SpCas9-h (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-h (no stop codon) pAGM1287
    pEPOR0SP0013 SpCas9-p (with stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-p pICH41308
    pEPOR0SP0009 SpCas9-p (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-p (no stop codon) pAGM1287
    pEPOR0SP0001 SpCas9-DE (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon) pAGM1287
    pEPOR0SP0014 SpCas9-KA (with stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-KA pICH41308
    pEPOR0SP0015 SpCas9-KA (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon) pAGM1287
    pEPOR0SP0016 Sp-eCas9 1.0 (with stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, Sp-eCas9 1.0 pICH41308
    pEPOR0SP0017 Sp-eCas9 1.0 (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon) pAGM1287
    pEPOR0SP0018 Sp-eCas9 1.1 (with stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, Sp-eCas9 1.1 pICH41308
    pEPOR0SP0019 Sp-eCas9 1.1 (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon) pAGM1287
    pEPOR0SP0011 Sp-xCas9 3.7 (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon) pAGM1287
    pEPOR0SP0020 SaCas9 (with stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SaCas9 pICH41308
    pEPOR0SP0021 SaCas9 (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, SaCas9 (no stop codon) pAGM1287
    pEPOR0SP0012 eSaCas9 (no stop codon) (Other) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level0 Golden Gate part: CDS, eSaCas9 (no stop codon) pAGM1287
    pICSL11023 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants pICH47742
    pEPOR1CB0002 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants pICH47742
    pEPOR1CB0009 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants pICH47742
    pEPOR1CB0011 2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants pICH47742
    pEPOR1CB0112 2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants pICH47742
    pEPOR1CB0015 2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) Patron Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766 Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants pICH47742
    p221z-CAS9p-t35s CAS9p (Other) Mähönen An inducible genome editing system for plants. Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 10.1038/s41477-020-0695-2. Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z
    p221z-CAS9p-TagRFP-t35s CAS9p-TagRFP (Other) Mähönen An inducible genome editing system for plants. Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 10.1038/s41477-020-0695-2. Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z
    pYPQ150-x3.7 pcoCas9-x3.7 Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized) pNJB91
    pGEL062 Hygromycin Zhang Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. For genome editing in plant by SpCas9 wild type pZHY988
    pGEL063 Hygromycin Zhang Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. For genome editing in plant by SpCas9 variant(SpCas9-NG) pZHY988
    pGEL065 Hygromycin Zhang Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. For genome editing in plant by SpCas9 variant(SpCas9-NGv1) pZHY988
    pFH13 SpCas9, wheat codon optimized Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 SpCas9, wheat codon optimized pICH41308
    pFH14 SaCas9, Arabidopsis codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 SaCas9, Arabidopsis codon optimized pICH41308
    pFH15 SaCas9, Arabidopsis codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 StCas9, Arabidopsis codon optimized pICH41308
    pFH16 FnCas12a, rice codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 FnCas12a, rice codon optimized pICH41308
    pFH17 LbCas12a, rice codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 LbCas12a, rice codon optimized pICH41308
    pFH22 xCas9 3.7 (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 xCas9 3.7 pICH41308
    pFH32 SpCas9-NG, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 SpCas9-NG, human codon optimized pICH41308
    pFH46 FnCas12a, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 FnCas12a, human codon optimized pICH41308
    pFH47 LbCas12a, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 LbCas12a, human codon optimized pICH41308
    pFH48 Csy4 (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 Csy4 pICH41308
    pFH76 ScCas9, wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 ScCas9, wheat codon optimized pICH41308
    pFH77 enCas9-PolI3M-TBD, wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 enCas9-PolI3M-TBD, wheat codon optimized pICH41308
    pRGEB32-BAR Basta ; BAR Hunter CRISPR/Cas9 vector optimized for Agrobacterium-mediated transformation of maize (with BAR selectable marker) (modified from pRGEB32) (unpublished) For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs. pRGEB32
    pENTR_Wheat_live_Cas9 Wheat_live_Cas9 (Synthetic) Kamoun Genome editing in wheat (unpublished) Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules. pENTR4
    pICSL11085_GG_Wheat_live_Cas9_for Wheat_live_Cas9 (Synthetic) Kamoun Genome editing in wheat (unpublished) Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids. pICH47742
    pICSL11086_GG_Wheat_live_Cas9_rev Wheat_live_Cas9 (Synthetic) Kamoun Genome editing in wheat (unpublished) Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids. pICH47742
    pFH23 ZmUBIp::SpCas9 (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt pICH47742
    pFH52 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt pICH47742
    pFH53 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At pICH47742
    pFH54 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At pICH47742
    pFH58 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt pICH47742
    pFH59 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt pICH47742
    pFH60 ZmUBIp::FnCas12a (rice codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt pICH47742
    pFH61 ZmUBIp::LbCas12a (rice codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt pICH47742
    pFH68 ZmUBIp::SpCas9-NG (human codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt pICH47742
    pFH80 ZmUBIp::xCas9 3.7:NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::xCas9 3.7:NOSt pICH47742
    pFH81 ZmUBIp::ScCas9 (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt pICH47742
    pFH82 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt pICH47742
    pARS3_MUbCAS9_GW Hygromycin Schaller Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870. T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA pARS3
  • Tag / Fusion Protein
    • HA
    • pARS3_MUbCAS9_MC Hygromycin Nimchuk Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870. T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA pARS3
  • Tag / Fusion Protein
    • HA
    • pFH24 SpCas9, wheat codon optimized, version 2 (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 SpCas9, wheat codon optimized (version 2) pICH41308
    pFH25 SpCas9, wheat codon optimized, version 3 (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 SpCas9, wheat codon optimized (version 3) pICH41308
    pFH66 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt pICH47742
    pFH67 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt pICH47742
    pG3GB411 CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3H-U3Ub CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3B-U3Ub CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3B-U6SC CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3K-U6SC CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3H-U6SC CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3B-U6EC1 CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3K-U6EC1 CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pG3H-U6EC1 CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pGreen3 CRISPR/Cas9 pGreen3
    pGB411 CRISPR/Cas9 (Synthetic) Chen A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. pCambia CRISPR/Cas9 pCambia
    pGGA_RT_Sa_Cas9 SaCAS9 (Synthetic) Theres Theres lab: Alternative Greengate/Goldengate modules (unpublished) Goldengate/Greengate module for genome editing, using the SaCAS9. pUC19
    pGEL077 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone pZHY988
    pGEL078 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone pZHY988
    pGEL079 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone pZHY988
    pGEL081 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone pZHY988
    pGEL082 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone pZHY988
    pGEL083 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone pZHY988
    pGEL085 Zhang Yong Zhang lab plasmids (unpublished) iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone pZHY988
    pGEL029 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 SpyCas9 RZ system for plant genome editing pZHY988
    pGEL031 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 SpyCas9 tRNA system for plant genome editing pZHY988
    pGEL033 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988
    pGEL050 Zhang Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front Plant Sci. 2019 Sep 20;10:1173. doi: 10.3389/fpls.2019.01173. eCollection 2019. Dual-mini35S driven Cas9-Csy4 system pTX152
    pGEL051 Zhang Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front Plant Sci. 2019 Sep 20;10:1173. doi: 10.3389/fpls.2019.01173. eCollection 2019. Dual-mini35S-enhancer driven Cas9-Csy4 system pTX152
    pRGEB-Cas9-VirD2 (PCO) Cas9-VirD2 (Synthetic) Hygromycin Mahfouz Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice. Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9. Expressing Cas9-VirD fusion under ubiquitin promoter pRGEB32
    pRGEB-VirD2-Cas9 VirD2-Cas9 (Synthetic) Hygromycin Mahfouz Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice. Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9. Expressing VirD-Cas9 fusion under ubiquitin promoter pRGEB32
    pYPQ166-iSpyMac iSpyMacCas9 Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs pYPQ165
    pAGM55261 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker pAGM4723
    pAGM55273 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker pAGM4723
    pAGM55285 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker pAGM37443
    pAGM55297 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker pAGM37443
    pAGM65879 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette pAGM35831
    pAGM65881 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation pAGM35831
    pAGM65893 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette pAGM37443
    pAGM65905 Marillonnet Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants BioRxiv 2020.04.03.023036 Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation pAGM37443

    Base Edit

    Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations.

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pMonAID_dCas9-PmCDA_Hyg_ALS SpCas9 (Other) 2x35S Hygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pMonAID_nCas9-PmCDA_Hyg_ALS SpCas9 (Other) 2x35S Hygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pDicAID_nCas9-PmCDA_NptII_Della SpCas9 (Other) PcUbi Kanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
    pDicAID_nCas9-PmCDA-2A-NptII_ETR SpCas9 (Other) PcUbi Kanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
    pHEE901 Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
    pHSE901 Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
    pBSE901 Neomycin (select with G418) Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
    pXSE901B Basta Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
    pXSE901BG Basta ; Glyphosate Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
    pB-dCas9-PBE NLS-APOBEC1-XTEN-dCas9-UGI-NLS Ubi Basta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pB-nCas9-PBE NLS-APOBEC1-XTEN-nCas9-UGI-NLS Ubi Basta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pdCas9-PBE NLS-APOBEC1-XTEN-dCas9-UGI-NLS Ubi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pH-dCas9-PBE NLS-APOBEC1-XTEN-dCas9-UGI-NLS Ubi Hygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pH-nCas9-PBE NLS-APOBEC1-XTEN-nCas9-UGI-NLS Ubi Hygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pnCas9-PBE NLS-APOBEC1-XTEN-nCas9-UGI-NLS Ubi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pJY-RpABE RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA cassette Hygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for ABE expression in Arabidopsis pKI1.1R
    pJY-RpABE-FT_INF RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting FT Hygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis pJY-RpABE
    pJY-RpABE-PDS3_IMS RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting PDS3 Hygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis pJY-RpABE
    pH-PABE-7-esgRNA wtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    pH-PABE-7-sgRNA wtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    PABE-1 NLS-wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-2 wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-3 wtTadA-TadA7.10-NLS-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-4 NLS-nCas9-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-5 NLS-nCas9-NLS-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-6 wtTadA-TadA7.10-nCas9-2*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-7 wtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    pYPQ255 rAPOBEC1-pcoCas9D10A-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ255-x3.7 rAPOBEC1-pcoCas9D10A_x3.7-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pYPQ256 pcoCas9D10A-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ256-x3.7 pcoCas9D10A_x3.7-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pGEL066 Hygromycin Zhang Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor pZHY988
  • Tag / Fusion Protein
    • 3xFlag (C terminal on backbone)
    		•
    pFH45 nSpCas9-ABE7.10 base changer, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nSpCas9-ABE7.10 base changer, human codon optimized pICH41308
    pFH55 nSpCas9-PmCDA, Arabidopsis codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nSpCas9-PmCDA, Arabidopsis codon optimized pICH41308
    pFH79 A3A-PBE, wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 A3A-PBE, wheat codon optimized pICH41308
    pFH92 nScCas9-ABE7.10, wheat/human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nScCas9-ABE7.10, wheat/human codon optimized pICH41308
    pFH70 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt pICH47742
    pFH84 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt pICH47742
    pFH93 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt pICH47742
    pGEL033 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988
    pYPQ265H hAID-ziSpyMacCas9(D10A)-UGI Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs pNJB91
    pYPQ266B ziSpyMacCas9(D10A)-PmCDA1-UGI Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs pNJB91
    pYPQ262m3 ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A) Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs pYPQ165

    Nick

    CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) Ubi1p, AtU6-26p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance pGreen-like binary vector
    pHSN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pDe-CAS9-D10A Cas9-D10A (Arabidopsis thaliana) PcUbi4-2 Kanamycin Puchta Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. based on pDe-CAS9, encodes Cas9 D10A nickase variant pPZP221
    pYPQ159 hSpCas9D10A (Human codon optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with hSpCas9D10A pNJB185-linker6
    pDGE76 pnos:nptII-tmos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII pVM_BGW
    pDGE77 pnos:PAT-tnos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA pVM_BGW
    pDGE78 pnos:nptII-tmos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII pVM_BGW
    pDGE79 pnos:PAT-tnos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA pVM_BGW
    pTX179 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants pTX172
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pMOD_A0201 AtCas9_D10A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0202 AtCas9_D10A nickase (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0208 AtCas9_D10A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0301 AtCas9_H840A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0302 AtCas9_H840A nickase (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0308 AtCas9_H840A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0601 Csy4-P2A-AtCas9_D10A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0608 Csy4-P2A-AtCas9_D10A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0701 Csy4-P2A-AtCas9_H840A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0708 Csy4-P2A-AtCas9_H840A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1210 TaCas9_D10A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1310 TaCas9_H840A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1610 Csy4-P2A-TaCas9_D10A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1710 Csy4-P2A-TaCas9_H840A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pAtCas9-D10A_1 AtCas9_D10A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9-D10A_2 AtCas9_D10A (nickase) (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
    pAtCas9-D10A_3 AtCas9_D10A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar pCAMBIA
    pAtCas9-H840A_1 AtCas9_H840A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9-H840A_2 AtCas9_H840A (nickase) (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
    pTaCas9-D10A_5 TaCas9_D10A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9-D10A_6 TaCas9_D10A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
    pTaCas9-H840A_5 TaCas9_H840A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9-H840A_6 TaCas9_H840A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
    pJG363 nAtCas9_D10A+gNt_R2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor) pTRANS_201
    pJG367 nAtCas9_H840A+gNt_R2+gNt_F2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor) pTRANS_201
    pJG370 nAtCas9_D10A+gRNActrl+donor (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor) pTRANS_201
    pJG382 nAtCas9_D10A+gNt_R2+gNt_F2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor) pTRANS_201
    pJG484 nTaCas9_H840A+gUbi8+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor) pTRANS_203
    pJG486 nTaCas9_H840A+gUbi1+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor) pTRANS_203
    pJG488 nTaCas9_H840A+gUbi8+gUbi1+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor) pTRANS_203
    pORFE0002 AtCAS9 nickase D10A (Other) Chevalier Chevalier Lab plasmids (unpublished) AtCAS9 nickase D10A module CDS1 pICH41308
    pORFE0014 AtCAS9 D10A without stop codon (Other) Chevalier Chevalier Lab plasmids (unpublished) AtCAS9 nickase D10A module CDS1ns pAGM1287
    pORFE1052 AtCAS9 D10A (Other) 2x35S Chevalier Chevalier Lab plasmids (unpublished) Overexpression of the AtCAS9 nickase in plant under 2x35S promoter pICH47742
    pFH91 nScCas9 (nickase), wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nScCas9 (nickase), wheat codon optimized pICH41308
    pFH109 ZmUBIp::nScCas9 (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt pICH47742

    Activate

    Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN6A11 dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic) Ubi1p, OsU3p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
    pHSN6A01 dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pYPQ152 pco-dCas9-VP64 (Plant codon-optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with pco-dCas9-VP64 pYPQ185-linker1
    pYPQ173 pco-dCas9-VP64-T2A-MS2-VP64 (Synthetic) Qi Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems. Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010. Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence unknown
    pEG302 5aa SunTag VP64 nog NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS Hygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag NtDRMcd nog NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS Hygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag NtDRMcd (noNLS) nog NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS Hygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide) pEG302
    pEG302 22aa SunTag VP64 nog NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS Hygromycin Jacobsen Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
    PV225 dCas9-AT-CBF1 (Other) Young Repurposing of Type I-E CRISPR-Cascade for gene activation in plants Nature Communications Biology dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays PHP17720
  • Tag / Fusion Protein
    • AT-CBF1 (C terminal on insert)
    • CBF1 AT4G25490, ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1
    pYPQ239A (dFnCas12a)-TV dFnCas12a-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system pYPQ167
    pYPQ292 (AaCas12b)-D570A-TV AaCas12b (D570A)-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system pYPQ167
    pYPQ292 (AaCas12b)-D570A-TV-MS2-TV AaCas12b (D570A)-TV-T2A-MCP-TV (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein pYPQ167
    pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR AaCas12b (D570A)-TV-T2A-MCP-VPR (Other) Qi CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020) CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein pYPQ167

    Interfere

    Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus.

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN6I11 dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic) Ubi1p, OsU3p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
    pHSN6I01 dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pEGB 35S:dCas9:Tnos (GB1191) dCas9 (Other) 35S Orzaez GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31. Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter pDGB3alpha2
    pYPQ153 pco-dCas9-3X(SRDX) (Plant codon-optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with pco-dCas9-3X(SRDX) pYPQ185-linker1
    pdCas9 (GB1079) Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other) Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions pUPD
    pEGB 35s:dCas:BRD:tNos (GB1172) dCas9:BRD (Synthetic) 35S Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor pDGB3alpha2
    pYPQ223 (dAsCpf1-SRDX) dAsCpf1-SRDX (Other) Qi A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid pYPQ221
    pYPQ233 (dLbCpf1-SRDX) LbCpf1-SRDX (Other) Qi A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid pYPQ221
    pMOD_A0402 AtCas9_dead (D10A + H840A) (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A0408 AtCas9_dead (D10A + H840A) (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A0801 Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A0802 Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A0808 Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A1410 TaCas9_dead (D10A + H840A) (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pMOD_A1810 Csy4-P2A-TaCas9_dead (D10A + H840A) (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
    pDIRECT_21B Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II pTRANS_210
    pDIRECT_25G Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II pTRANS_250d
    pDIRECT_26G Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar pTRANS_260d
    pAtCas9-dead_1 AtCas9_D10A+H840A (dead) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9-dead_2 AtCas9_D10A+H840A (dead) (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II pCAMBIA
    pAtCas9-dead_3 AtCas9_D10A+H840A (dead) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar pCAMBIA
    pTaCas9-dead_5 TaCas9_D10A+H840A (dead) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9-dead_6 TaCas9_D10A+H840A (dead) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar pCAMBIA
    pHdzCas9-KRAB CaMV35S Hygromycin Ng CRISPRi mediated phosphoenolpyruvate carboxylase regulation to enhance the production of lipid in Chlamydomonas reinhardtii. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30619-3. doi: 10.1016/j.biortech.2017.04.111. Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance pHdzCas9-KRAB
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)
    		•
    pORFE0003 AtDeadCAS9 D10A H840A (Other) Chevalier Chevalier Lab plasmids (unpublished) AtDeadCAS9 D10A H840A module CDS1 pICH41308
    pORFE0005 AtDeadCAS9 D10A H840A without stop codon (Other) Chevalier Chevalier Lab plasmids (unpublished) AtDeadCAS9 D10A H840A without stop codon module CDS1ns pAGM1287
    p221z-dCAS9p-t35s dCAS9p (Other) Mähönen An inducible genome editing system for plants. Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 10.1038/s41477-020-0695-2. Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z

    Empty gRNA Expression Vectors

    Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids.

    Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI
    pICSL01009::AtU6p aU6 BsaI none S. pyogenes Kamoun
    pUC119-gRNA U6 PCR template none S. pyogenes Sheen
    pRGEB31 rice snoRNA U3 BsaI none S. pyogenes Yang
    pBUN6A11 OsU3 BsaI yes, activate S. pyogenes Bar Chen
    pBUN6I11 OsU3 BsaI yes, interfere S. pyogenes Bar Chen
    pBUN501 AtU6-26 BsaI yes, nick S. pyogenes Bar Chen
    pCBC-MT2T3 see paper BsaI none S. pyogenes Chen
    pCBC-MT3T4 see paper BsaI none S. pyogenes Chen
    pBUN421 TaU3 BsaI yes, cut S. pyogenes Bar Chen
    pU3-gRNA OsU3 AarI none S. pyogenes Gao
    pZmU3-gRNA maize U3 none S. pyogenes Gao
    pU6-gRNA wheat U6 BbsI none S. pyogenes Gao
    pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes Stupar
    pBUE411 OsU3 yes, cut S. pyogenes Basta Chen
    pHUE411 OsU3 yes, cut S. pyogenes Hyg Chen
    pHAtC U6 AarI yes, cut S. pyogenes Hygro Kim
    pBAtC U6 AarI yes, cut S. pyogenes Basta Kim
    pHEE401 U6-26p BsaI yes, cut S. pyogenes Hygro Chen
    pHEE401E U6-26p BsaI yes, cut S. pyogenes Hygro Chen
    pHDE-35S-Cas9-mCherry-UBQ U6-26 PmeI yes, cut S. pyogenes Hygro, mCherry Zhao
    pCBC-DT1T1 see paper BsaI none S. pyogenes Chen
    pRGEB32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang
    pHSE401 AtU6-26 yes, cut S. pyogenes Hyg Chen
    pBUN411 OsU3 BsaI yes, cut S. pyogenes Bar Chen
    pHSN6A01 AtU6-26 BsaI yes, activate S. pyogenes Hyg Chen
    pHSN6I01 AtU6-26 BsaI yes, interfere S. pyogenes Hyg Chen
    pHSN501 AtU6-26 BsaI yes, nick S. pyogenes Hyg Chen
    pCBC-DT3T4 see paper BsaI none S. pyogenes Chen
    pCBC-MT1T2 see paper BsaI none S. pyogenes Chen
    pRGE32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang
    pKSE401 AtU6-26 yes, cut S. pyogenes Kan Chen
    pRGE31 rice snoRNA U3 BsaI none S. pyogenes Yang
    pUC gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott
    pCBC-DT2T3 see paper BsaI none S. pyogenes Chen
    pHSN401 AtU6-26 BsaI yes, cut S. pyogenes Hyg Chen
    pYPQ141-ZmUbi-RZ-As Maize ubiquitin 1 none As Cpf1 Qi
    pKEE401 yes, cut S. pyogenes Neomycin Chen

    Do you have suggestions for other plasmids that should be added to this list?

    Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!