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CRISPR Plasmids: Nick

Browse CRISPR Plasmids

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CRISPR Resources

CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).



Browse, sort, or search the tables below for CRISPR nickase plasmids.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.

Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
hCas9_D10A Cas9_D10A CMV Church RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3. Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering pcDNA3.3-TOPO
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 (D10A) nickase Puromycin Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
    		•
    pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) humanized S. pyogenes Cas9 (D10A) nickase Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
    		•
    pCas9D10A_GFP Cas9D10A-2A-GFP (Synthetic) CAG Musunuru Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs. Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 10.1016/j.stem.2013.03.006. Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells pCAG
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
    		•
    pSpCas9n(BB)-2A-GFP (PX461) hSpCas9n (Synthetic) Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX461
    pSpCas9n(BB)-2A-Puro (PX462) hSpCas9n-2A-Puro (Synthetic) Cbh Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987 PX462
    pSpCas9n(BB) (PX460) hSpCas9n (Synthetic) Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes PX460
    pST1374-N-NLS-flag-linker-Cas9-H840A Cas9 nickase CMV Blasticidin Huang Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. Cas9 nickase optimized for nuclear import. Addgene 44758
    pST1374-N-NLS-flag-linker-Cas9-D10A Cas9 nickase CMV Blasticidin Huang Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. Cas9 nickase optimized for nuclear import. Addgene 44758
    pCAG-T3-hCasD10A-pA Codon-optimized Cas9 D10A (Synthetic) CAG promoter Fujii Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 10.1016/j.bbrc.2014.01.141. Expresses Cas9-nickase in mammalian cells and zygotes. pCAGGS
    pCAG-hCas9D10A hCas9D10A (Other) CAG Neomycin (select with G418) Hatada pCAG-hCas9D10A (unpublished) The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter pEGFP-N1
    pNW3 Csy4-T2A-Cas9n (D10A) CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. Csy4 and Cas9 D10A nickase expression plasmid pCAG-CFP
    N-Terminal Split Cas9 D10A Nickase with GyrA intein D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein (Homo sapiens) CBh Bao Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777. Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production Plasmid 42235: pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
    pHL-EF1a-SphcCas9(D10A)-iP-A CRISPR Cas9 D10A (Other), puromycin resistance gene (Other) Puromycin Hotta Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene. pHL-EF1a-GW-iP-A
    pXCas9H840A CBh and U6 Conklin Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Scientific Reports 6, Article number: 23549 (2016) Dual Expression Vector for Cas9 H840A nickase and gRNA pUC ori vector
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)
    		•
    c3GIC9n humanized S. Pyogenes Cas9 (D10A) (Other) TRE3G Dow Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 2015 Feb 18. col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes col1a1 Flp-in targeting construct
    pSpCas9n(BB)-2A-Puro (PX462) V2.0 hSpCas9n-2A-Puro (Synthetic) Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX462
    lentiCas9n(D10A)-Blast Cas9 (Synthetic), Blasticidin resistance EFS-NS, EFS-NS Blasticidin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW
    PX429 SpCas9 N863A nickase SpCas9 N863A nicking mutant (Synthetic) Cbh Zhang Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. SpCas9 N863A nickase PX165
    TC9nickpAB Cas9 nickase (Other) T7 Degenhardt Degenhardt lab Cas9 plasmids (unpublished) used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI PCR2-1 TOPO
    pCR1054 T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS Corn Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481. Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS SPynCas9
    pCR1055 T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS Corn Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481. Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS SPynCas9
    AIO-GFP Jackson CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing All-in-One
    AIO-mCherry Jackson CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing All-in-One
    AIO-Puro Cbh Puromycin Jackson CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing All-in-One_Nickase-D10A
  • Tag / Fusion Protein
    • Puromycin resistant marker
    • pBSKΔB-CBh-Cas9n-pA SpCas9n (Other) Ochiai Role of dynamic nuclear deformation on genomic architecture reorganization. PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 10.1371/journal.pcbi.1007289. eCollection 2019 Sep. Expresses Cas9 nickase in mammalian cells pBSK
    pDG330 humanized CRISPR associated protein 9 Nickase (Other), U6-gRNA scaffold 1, U6-gRNA scaffold 2 CBh, U6, U6 Thomas Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system. pX330-U6-Chimeric_BB-CBh-hSpCas9
    pDG335 humanized CRISPR associated protein 9 Nickase (Other), U6-gRNA scaffold 1, U6-gRNA scaffold 2 CBh, U6, U6 Thomas Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
    pDG461 humanized CRISPR associated protein 9 Nickase (Other), U6-gRNA scaffold 1, U6-gRNA scaffold 2 CBh, U6, U6 EGFP Thomas Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts. pSpCas9n(BB)-2A-GFP (PX461)
    pDG462 humanized CRISPR associated protein 9 Nickase (Other), U6-gRNA scaffold 1, U6-gRNA scaffold 2 CBh, U6, U6 Puromycin Thomas Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts. pSpCas9n(BB)-2A-Puro (PX462) V2.0
    pLL8 lenti-D10Acas9-3xflag-blast D10Acas9-3xflag-blast (Synthetic) EF1a Blasticidin Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE) lenti dCAS-VP64_Blast
    pOTTC1124 - pAAV MeCP2 SpCas9(D10A) SpCas9 (Synthetic) Mecp2 Harvey Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter pX551

    Bacteria

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pMJ825 Cas9 T7 Doudna A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012 Jun 28. pEC-K-MBP
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
    		•
    pMJ826 Cas9 T7 Doudna A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012 Jun 28. pEC-K-MBP
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
    		•
    pMCSG7-D16A-NmeCas9 pMCSG7-D16A-NmeCas9 (Other) Sontheimer DNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site pMCSG7
    pMCSG7-H588A-NmeCas9 pMCSG7-H588A-NmeCas9 (Other) T7 Sontheimer DNase H Activity of Neisseria meningitidis Cas9. Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020. bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site pMCSG7
    pNICKclos2.0 Cas9 nickase (Other), sgRNA to xylR (Synthetic) Pthl, Pj23119 Erythromycin in clostridium Yang CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052 pXY1
    pNICKclos1.0 Cas9 nickase (Other), sGRNA to pyrE ptb, j23119 Erythromycin in clostridium Yang CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824 pIMP1-ptb
    pCas9(D10A) Cas9 D10A (Synthetic) pTeto Wang Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases. ACS Synth Biol. 2015 Nov 20;4(11):1217-25. doi: 10.1021/acssynbio.5b00132. Epub 2015 Oct 25. Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A pWTCas9
    pLCNICK Cas9 nickase (Other), sgRNA, homology arms of LC2W_2179 Yang CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Appl Environ Microbiol. 2017 Sep 1. pii: AEM.01259-17. doi: 10.1128/AEM.01259-17. Genome editing for Lactobacillus casei Lc2W pCas

    Drosophila

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pDCC5 Cas9-D10A (Synthetic) hsp70Bb Duchek Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila. G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 10.1534/g3.114.014126. Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette pHW

    Plant

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) Ubi1p, AtU6-26p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance pGreen-like binary vector
    pHSN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pDe-CAS9-D10A Cas9-D10A (Arabidopsis thaliana) PcUbi4-2 Kanamycin Puchta Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. based on pDe-CAS9, encodes Cas9 D10A nickase variant pPZP221
    pYPQ159 hSpCas9D10A (Human codon optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with hSpCas9D10A pNJB185-linker6
    pDGE76 pnos:nptII-tmos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII pVM_BGW
    pDGE77 pnos:PAT-tnos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA pVM_BGW
    pDGE78 pnos:nptII-tmos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII pVM_BGW
    pDGE79 pnos:PAT-tnos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI Stuttmann Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA pVM_BGW
    pTX179 Hygromycin Voytas A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19. Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants pTX172
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pMOD_A0201 AtCas9_D10A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0202 AtCas9_D10A nickase (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0208 AtCas9_D10A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0301 AtCas9_H840A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0302 AtCas9_H840A nickase (Synthetic) AtUbi10 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0308 AtCas9_H840A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0601 Csy4-P2A-AtCas9_D10A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0608 Csy4-P2A-AtCas9_D10A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0701 Csy4-P2A-AtCas9_H840A nickase (Synthetic) 35S Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A0708 Csy4-P2A-AtCas9_H840A nickase (Synthetic) EC1.2 Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1210 TaCas9_D10A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1310 TaCas9_H840A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1610 Csy4-P2A-TaCas9_D10A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
    pMOD_A1710 Csy4-P2A-TaCas9_H840A nickase (Synthetic) ZmUbi Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
    pAtCas9-D10A_1 AtCas9_D10A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9-D10A_2 AtCas9_D10A (nickase) (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
    pAtCas9-D10A_3 AtCas9_D10A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar pCAMBIA
    pAtCas9-H840A_1 AtCas9_H840A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
    pAtCas9-H840A_2 AtCas9_H840A (nickase) (Synthetic) Kanamycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
    pTaCas9-D10A_5 TaCas9_D10A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9-D10A_6 TaCas9_D10A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
    pTaCas9-H840A_5 TaCas9_H840A (nickase) (Synthetic) Hygromycin Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
    pTaCas9-H840A_6 TaCas9_H840A (nickase) (Synthetic) Basta Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
    pJG363 nAtCas9_D10A+gNt_R2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor) pTRANS_201
    pJG367 nAtCas9_H840A+gNt_R2+gNt_F2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor) pTRANS_201
    pJG370 nAtCas9_D10A+gRNActrl+donor (Synthetic) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor) pTRANS_201
    pJG382 nAtCas9_D10A+gNt_R2+gNt_F2+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor) pTRANS_201
    pJG484 nTaCas9_H840A+gUbi8+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor) pTRANS_203
    pJG486 nTaCas9_H840A+gUbi1+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor) pTRANS_203
    pJG488 nTaCas9_H840A+gUbi8+gUbi1+donor (Other) Voytas A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor) pTRANS_203
    pORFE0002 AtCAS9 nickase D10A (Other) Chevalier Chevalier Lab plasmids (unpublished) AtCAS9 nickase D10A module CDS1 pICH41308
    pORFE0014 AtCAS9 D10A without stop codon (Other) Chevalier Chevalier Lab plasmids (unpublished) AtCAS9 nickase D10A module CDS1ns pAGM1287
    pORFE1052 AtCAS9 D10A (Other) 2x35S Chevalier Chevalier Lab plasmids (unpublished) Overexpression of the AtCAS9 nickase in plant under 2x35S promoter pICH47742
    pFH91 nScCas9 (nickase), wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nScCas9 (nickase), wheat codon optimized pICH41308
    pFH109 ZmUBIp::nScCas9 (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt pICH47742

    Yeast

    ID Plasmid Purpose Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    79616 pRS415_pGal-nCas9(H840A) Expresses nCas9(H840A) in yeast cells SpCas9 (Other) pGal1 LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(H840A) in yeast cells pRS415
    79617 pRS315e_pGal-nCas9(D10A)-PmCDA1 Expresses nCas9(D10A)-PmCDA1 in yeast cells SpCas9 (Other) pGal1 LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(D10A)-PmCDA1 in yeast cells pRS315
    79618 pRS415_pGal-nCas9(D10A) Expresses nCas9(D10A) in yeast cells SpCas9 (Other) pGal1 LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(D10A) in yeast cells pRS415


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