|
p414-TEF1p-Cas9-CYC1t |
Human Optimized S. pyogenes Cas9 (Homo sapiens) |
TEF1 promoter |
TRP1 |
Church
|
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.
Nucleic Acids Res
|
p414
|
|
p415-GalL-Cas9-CYC1t |
Human Optimized S. pyogenes Cas9 (Homo sapiens) |
GalL |
LEU2 |
Church
|
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.
Nucleic Acids Res
|
p415
|
|
pACT2-CAS9c |
Cas9c (Synthetic) |
yeast ADH1 promoter |
LEU2 |
Zhao
|
Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing.
J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
|
Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
pACT2
|
|
pMZ222 |
Cas9 (Other) |
adh1 |
LEU2 |
Zaratiegui
|
Implementation of the CRISPR-Cas9 system in fission yeast.
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
|
Constitutive expression of humanized Cas9
pART1
|
|
pMZ374 |
Cas9 (Other), rrk1:sgRNA (Other) |
adh1, rrk1 |
ura4 |
Zaratiegui
|
Implementation of the CRISPR-Cas9 system in fission yeast.
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
|
Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
pMZ283
|
|
pCT |
iCas9 (Other), tracrRNA (Other) |
TEF1p, RPR1p |
LEU2 |
Zhao
|
Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.
ACS Synth Biol. 2014 Sep 19.
|
Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
pRS415
|
|
pCRCT |
iCas9 (Other), tracrRNA (Other) |
TEF1p, RPR1p |
URA3 |
Zhao
|
Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.
ACS Synth Biol. 2014 Sep 19.
|
Plasmid encoding iCas9, tracrRNA and crRNAs
pRS426
|
|
Cas9-NAT |
Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette |
|
Nourseothricin(clonNat) |
Jin
|
Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
|
Cas9 expression cassette carried by a yeast single-copy episome plasmid
pRS414-TEF1p-Cas9-CYC1t
|
|
pML104 |
Cas9 (Saccharomyces cerevisiae), single guide RNA expression cassette (Saccharomyces cerevisiae) |
pTDH3, pSNR52 |
URA3 |
Wyrick
|
New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
pRSII426
|
|
pML107 |
Cas9 (Saccharomyces cerevisiae), single guide RNA expression cassette (Saccharomyces cerevisiae) |
pTDH3 (aka GAP promoter), pSNR52 |
LEU2 |
Wyrick
|
New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
pRSII425
|
|
pJH001 |
Cas9 (Saccharomyces cerevisiae), Empty |
|
LEU2 |
Wyrick
|
New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
High copy Cas9 expression vector with LEU2 marker for yeast transformation
pRSII425
|
|
p415-PtrpC-Cas9-TtrpC-CYC1t |
humanized Cas9 (Homo sapiens) |
|
LEU2 |
Hong
|
Efficient gene editing in Neurospora crassa with CRISPR technology
Fungal Biology and Biotechnology 2015, 2:4
|
Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
pRS415
|
|
pCRISPRyl |
Codon optimized Cas9 from S. pyogenes (Synthetic), sgRNA expression cassette (Synthetic) |
UAS1B8-TEF(136), SCR1'-tRNA |
LEU2 |
Wheeldon
|
Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
ACS Synth Biol. 2015 Dec 29.
|
CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
pUC19
|
|
pMZ376 |
Cas9 (Other), rrk1:sgRNA (Other) |
adh1, rrk1 |
Neomycin (select with G418) |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with KanMX marker
pMZ374
|
|
pMZ377 |
Cas9 (Other), rrk1:sgRNA (Other) |
adh1, rrk1 |
LEU2 |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with LEU2 marker
pMZ374
|
|
pMZ379 |
Cas9 (Other), rrk1:sgRNA (Other) |
adh1, rrk1 |
nourseothricin |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with NatMX marker
pMZ374
|
|
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) |
Cas9 (Other) |
|
Gentamicin |
Borodina
|
EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance
pRS414
|
|
pRS416gT-GalL-Cas9 |
Cas9 (Synthetic), guide RNA (gRNA) (Synthetic), Tetracycline Repressor (Other) |
GalL, RPR1 promoter with TetO site, GPM1 promoter |
URA3 |
Davis
|
Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.
Nucleic Acids Res. 2016 May 19. pii: gkw417.
|
pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
pRS416
|
|
pRCC-K |
Cas9 (Other), empty gRNA cassette (Synthetic) |
ROX3, SNP52p |
Neomycin (select with G418) |
Boles
|
Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
pRS42K
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
|
pRCC-N |
Cas9 (Other), empty gRNA cassette (Synthetic) |
ROX3, SNP52p |
Nourseothricin |
Boles
|
Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
pRS42N
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
|
pDuRCC-K |
Cas9 (Other), empty gRNA cassette (Synthetic), empty gRNA cassette (Synthetic) |
ROX3, SNP52p, SNP52p |
Neomycin (select with G418) |
Boles
|
Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
pRS42K
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
|
pDuRCC-N |
Cas9 (Other), empty gRNA cassette (Synthetic), empty gRNA cassette (Synthetic) |
ROX3, SNP52p, SNP52p |
Nourseothricin |
Boles
|
Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
pRS42N
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
|
pML104-HygMx4 |
HphMX4 (Other) |
|
Hygromycin |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
pRSII426
|
|
pML104-KanMx4 |
KanMX4 (Other) |
|
Neomycin (select with G418) |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
pRSII426
|
|
pML104-NatMx3 |
NatMx3 (Other) |
|
nourseothricin (nat) |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
pRSII426
|
|
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) |
Cas9 (Other) |
|
G418 |
Borodina
|
CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains
Metab Eng Commun 2015
|
Cas9-carrying vector with kanMX marker
p414-TEF1p-Cas9-CYC1t
|
|
pCRISPRyl_AXP |
Cas9 |
UAS1B8-TEF(136) |
LEU2 |
Wheeldon
|
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at AXP locus in Y. lipolytica
pUC19
|
|
pCRISPRyl_XPR2 |
Cas9 |
UAS1B8-TEF(136) |
LEU2 |
Wheeldon
|
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at XPR2 locus in Y. lipolytica
pUC19
|
|
pCRISPRyl_A08 |
Cas9 |
UAS1B8-TEF(136) |
LEU2 |
Wheeldon
|
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at A08 locus in Y. lipolytica
pUC19
|
|
pCRISPRyl_D17 |
Cas9 |
UAS1B8-TEF(136) |
LEU2 |
Wheeldon
|
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at D17 locus in Y. lipolytica
pUC19
|
|
pCRISPRyl_MFE1 |
Cas9 |
UAS1B8-TEF(136) |
LEU2 |
Wheeldon
|
Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at MFE1 locus in Y. lipolytica
pUC19
|
|
pJK-caCas9-NatMX-Neut5L |
|
|
Nourseothricin |
Church
|
A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus
pJK1027
|
|
pJK-caCas9-NatMX-Neut5L-Ade2 drive |
Ade2 gene drive (Other) |
|
Nourseothricin |
Church
|
A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus
pJK1027
|
|
pJK-caCas9-NatMX-Neut5L-Leu2 drive |
Leu2 gene drive (Other) |
|
|
Church
|
A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus
pjk1027
|
|
pWS158 |
Cas9 (Saccharomyces cerevisiae) |
|
URA3 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - URA3
2 micron
|
|
pWS171 |
Cas9 (Saccharomyces cerevisiae) |
|
LEU2 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - LEU2
2 micron
|
|
pWS172 |
Cas9 (Saccharomyces cerevisiae) |
|
HIS3 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - HIS3
2 micron
|
|
pWS173 |
Cas9 (Saccharomyces cerevisiae) |
|
Kanamycin (select with G418) |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - KanR
2 micron
|
|
pWS174 |
Cas9 (Saccharomyces cerevisiae) |
|
Nourseothricin |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - NatR
2 micron
|
|
pWS175 |
Cas9 (Saccharomyces cerevisiae) |
|
Hygromycin |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - HygR
2 micron
|
|
pADH99 |
US_HIS1, FRT, pENO1, C. albicans Cas9, pMAL2, FLP, NAT 1 of 2 |
|
Nourseothricin (NAT) |
Hernday
|
An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
pUC19
|
|
pADH137 |
C. albicans LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 |
|
Nourseothricin (NAT) |
Hernday
|
An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
pUC19
|
|
pADH140 |
C. maltosa LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 |
|
Nourseothricin (NAT) |
Hernday
|
An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
pUC19
|
|
pDHt/sk-PC |
Cas9 (Other) |
Ppdc |
Hygromycin |
Zhou
|
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei
pDHt/sk
|
|
pDHt/sk-PE |
Cas9-eGFP (Other) |
Ppdc |
Hygromycin |
Zhou
|
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei
pDHt/sk
Tag
/ Fusion Protein- EGFP (C terminal on insert)
|
|
pDHt/sk-CC |
Cas9 (Other) |
Pcbh1 |
Hygromycin |
Zhou
|
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei
pDHt/sk
|
|
pDHt/sk-CE |
Cas9-eGFP (Other) |
Pcbh2 |
Hygromycin |
Zhou
|
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
pDHt/sk
Tag
/ Fusion Protein- EGFP (C terminal on insert)
|
|
pYZ033 |
|
|
Spura4 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
pMZ374
|
|
pYZ146 |
gRNA targeting Sp.leu1 (Schizosaccharomyces pombe) |
|
Spura4 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe
pYZ033
leu1 SPBC1A4.02c
|
|
pYZ164 |
gRNA targeting Sp.his3 (Schizosaccharomyces pombe) |
|
Spura4 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA his3 plasmid for his3 deletion in S. pombe
pYZ033
his3 SPBC11B10.02c
|
|
pYZ173 |
gRNA targeting Sp.lys9 (Schizosaccharomyces pombe) |
|
Spura4 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe
pYZ033
SPBC3B8.03 SPBC3B8.03
|
|
pDB4279 |
Cas9 (Other) |
|
|
Du
|
A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A control plasmid containing the intact bsdMX marker
pMZ374 (Addgene 59896)
|
|
pDB4280 |
Cas9 (Other) |
|
|
Du
|
A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
pMZ374 (Addgene 59896)
|
|
pDB4281 |
Cas9 (Other) |
|
|
Du
|
A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
pMZ374 (Addgene 59896)
|
|
pIW447-CRISPR XYL2 |
Codon optimized Cas9 (Synthetic), sgRNA expression cassette (Synthetic) |
|
URA3 |
Wheeldon
|
CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus.
Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.
|
K. marxianus CRISPR Plasmid for XYL2 editing
pJSK316-GPD
|
|
pIW601-KmCRISPR |
Codon optimized Cas9 (Synthetic), sgRNA expression cassette (Synthetic) |
|
URA3 |
Wheeldon
|
CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus.
Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.
|
K. marxianus CRISPR Plasmid for sgRNA cloning
pJSK316-GPD
|
|
bRA89 |
|
|
Hygromycin |
Haber
|
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker
pRS316
|
|
bRA90 |
|
|
LEU2 |
Haber
|
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker
pRS316
|
|
bRA66 |
|
|
Hygromycin |
Haber
|
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
GAL1 driven Cas9. gRNA can be cloned into BplI sites.
pRS316
|
|
bRA77 |
|
|
LEU2 |
Haber
|
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
GAL1 driven Cas9. gRNA can be cloned into BplI sites.
pRS316
|
|
pJH2970 |
|
|
HIS3 |
Haber
|
Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
pRS316
|
|
pJH2971 |
|
|
Neomycin (select with G418) |
Haber
|
Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
pRS316
|
|
pJH2972 |
|
|
URA3 |
Haber
|
Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
pRS316
|
|
pUDP004 |
|
ScTDH3 |
amdS (acetamidase gene conferring growth on acetamide) |
Daran
|
CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
not applicable
|
|
pUDP012 |
HH-gRNA-HDV targetting SeILV6 in S. pastorianus (Saccharomyces cerevisiae) |
ScTDH3, ScTDH3 |
amdS (acetamidase gene conferring growth on acetamide) |
Daran
|
CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
pUDP004
|
|
pUDP044 |
polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus (Saccharomyces cerevisiae) |
ScTDH3, ScTDH3 |
amdS (acetamidase gene conferring growth on acetamide) |
Daran
|
CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
pUDP004
|
|
pCSN061 |
S. pyogenes Cas9 codon optimized for expression in S. cerevisiae., KanMX marker expression cassette. (Other) |
Heterologous promoter from K. lactis (KLLA0F20031g), Heterologous TEF1 promoter from A. gossypii. |
TRP1 ; KanMX |
Verwaal
|
CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
NEWENTRY
|
|
pCSN067 |
Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae. (Other), KanMX marker expression cassette. (Other) |
Heterologous promoter from K. lactis (KLLA0F20031g)., Heterologous TEF1 promoter from A. gossypii. |
TRP1 ; KanMX |
Verwaal
|
CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
|
|
pCSN068 |
Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae. (Other), KanMX marker expression cassette. (Other) |
Heterologous promoter from K. lactis (KLLA0F20031g)., Heterologous TEF1 promoter from A. gossypii. |
TRP1 ; KanMX |
Verwaal
|
CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
|
|
pYZ292 |
|
|
LEU2 ; Sc |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - ScLEU2 marker
pYZ292
|
|
pYZ293 |
|
|
Sp leu1 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp leu1 marker
pYZ293
|
|
pYZ294 |
|
|
kanMX |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - KanMX marker
pYZ294
|
|
pYZ300 |
|
|
Sp his3 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp his3 marker
pYZ300
|
|
pYZ301 |
|
|
Sp lys9 |
Boeke
|
Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp lys9 marker
pYZ301
|
|
pUDE731 |
Fncpf1 (Other) |
TEF1 |
URA3 |
Daran
|
FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae.
Nucleic Acids Research (2017), gkx1007
|
S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p
pMEL10
|
|
pUDC175 |
Fncpf1 (Other) |
TEF1 |
TRP1 |
Daran
|
FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae.
Nucleic Acids Research (2017), gkx1007
|
S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter
p414TEF1
|
|
pUDP002 |
hph (Other), Spcas9 D147Y P411T (Other) |
Ashbya gossypii (Eremothecium gossypii) TEF1, Arxula adeninivorans TEF1 |
Hygromycin |
Daran
|
Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.
FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012.
|
Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
not applicable
|
|
BB3cN_pGAP_23*_pLAT1_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
NTC |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
Episomal ARS/CEN Backbone
|
|
BB3cH_pGAP_23*_pLAT1_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
Hygromycin |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
Episomal ARS/CEN Backbone
|
|
BB3cK_pGAP_23*_pLAT1_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
Episomal ARS/CEN Backbone
|
|
BB3cK_pGAP_23*_pTEF_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
Episomal ARS/CEN Backbone
|
|
BB3cK_pGAP_23*_pPFK300_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
Episomal ARS/CEN Backbone
|
|
BB3cN_pGAP_23*_pPFK300_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
NTC |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
Episomal ARS/CEN Backbone
|
|
BB3cH_pGAP_23*_pPFK300_Cas9 |
HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) |
|
Hygromycin |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
Episomal ARS/CEN Backbone
|
|
pCfB4906 |
Cas9 (Other) |
|
Hygromycin |
Borodina
|
EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.
Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
|
EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance
Unknown
|
|
pCfB6364 |
Cas9 (Other) |
|
D-serine marker; enables growth on D-serine |
Borodina
|
EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.
Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
|
EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance
Unknown
|
|
pHCas9-Nours |
Cas9 (Other) |
TEF1 |
URA3 |
Yang
|
Unraveling the genetic basis of fast l-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae.
Biotechnol Bioeng. 2018 Sep 10. doi: 10.1002/bit.26827.
|
Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
pSH47
|
|
pV1025 |
CaCas9 (Synthetic) |
|
Ampicillin |
Fink
|
A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
pV941
|
|
pV1093 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
clonNAT |
Fink
|
A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
pV1088/pV1090
|
|
pV1200 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
Ampicillin |
Fink
|
A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
pV1025/pV1093
|
|
pV1393 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
Ampicillin |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
pV1390
|
|
pV1524 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
Ampicillin |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
pV1393
|
|
pV1539 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
Ampicillin |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
Genedrive entry vector for Candida albicans
pV1524
|
|
pV1326 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
URA3 ; clonNAT |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
|
pV1382 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
URA3 ; clonNAT |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
|
pV1464 |
CaCas9/sgRNA-BsmBI stuffer (Synthetic) |
|
URA3 ; clonNAT |
Fink
|
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
N. castellii Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
|
pZS157 CRISPEY RT/Cas9 |
SpCas9, Ec86-RT (Other) |
GAL1-GAL10 |
HIS3 |
Fraser
|
Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing.
Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 10.1016/j.cell.2018.08.057.
|
Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression
pRS403
|
|
eGFP L202 gRNA |
eGFP L202 gRNA (Other) |
U6 |
|
Harris
|
A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.
MLM3636
|
|
eGFP L138 gRNA |
eGFP L138 gRNA (Other) |
U6 |
|
Harris
|
A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.
MLM3636
|
|
pAH235 |
humanized Streptococcus pyogenes Cas9 (Synthetic) |
nmt41 |
S.pombe ura4 |
Tanaka
|
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Cas9 expression controlled by the nmt41 promoter
pSLF273
|
|
pAH237 |
gRNA cassette (Schizosaccharomyces pombe), humanized Streptococcus pyogenes Cas9 (Schizosaccharomyces pombe) |
S.pombe rrk1, nmt41 |
LEU2 |
Tanaka
|
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
pAH233
|
|
pAH243 |
humanized Streptococcus pyogenes Cas9 (Schizosaccharomyces pombe), gRNA expression module (Schizosaccharomyces pombe) |
nmt41, S.pombe rrk1 |
S.pombe ura4 |
Tanaka
|
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
pSLF273
|
|
pUCC001 |
HH-BsaI (Synthetic) |
TDH3 promoter from S.cerevisiae (already present in backbone) |
Hygromycin |
Morrissey
|
Biological Parts for Kluyveromyces marxianus Synthetic Biology
Front. Bioeng. Biotechnol., 07 May 2019
|
Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
pUDP002
|
|
NM9-SpCas9-NLS3 |
SpCas9-NLS3 (Other) |
pPGK1 |
Neomycin (select with G418) |
Zhao
|
Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides.
Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001.
|
Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter
pCAMBRIA
|
|
pSDMA65 |
Hygromycin (HYG) (Synthetic) |
ACT1 |
Hygromycin |
Fraser
|
Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
(unpublished)
|
Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
pBlueScript II SK(-)
|
|
JME4390 |
|
|
LEU2 |
Rossignol
|
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4.
|
LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate
pSB1A3
|
|
JME4393 |
|
|
LYS5 |
Rossignol
|
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4.
|
LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate
pSB1A3
|
|
JME4472 |
|
|
URA3 |
Rossignol
|
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4.
|
URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate
pSB1A3
|
|
JME4580 |
|
|
Hygromycin |
Rossignol
|
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4.
|
HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate
pSB1A3
|
|
JME4599 |
|
|
Nourseothricin |
Rossignol
|
A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity.
Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4.
|
NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate
pSB1A3
|
|
pCP-tRNA |
cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter (Synthetic) |
|
SAT1 (resistance to Nourseothricin) |
Butler
|
Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species.
mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 10.1128/mSphere.00125-19.
|
CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
pUC57
|
|
pCT-tRNA |
cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter (Synthetic) |
|
SAT1 (resistance to Nourseothricin) |
Butler
|
Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species.
mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 10.1128/mSphere.00125-19.
|
CRISPR-Cas9 system for genetic manipulation of Candida tropicalis
pAYCU268
|
|
pAEF5 |
|
|
Hygromycin |
Fischer
|
Yeast Protocols 4th Edition--A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9
(unpublished)
|
Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
pGangZhao110
|
|
pCAS9i |
|
GAL1 (Cas9), pSNR52 (gRNA) |
URA3 |
Bertl
|
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018.
|
Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|
|
pCAS9c |
|
TEF1 (Cas9), pSNR52 (gRNA) |
URA3 |
Bertl
|
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018.
|
Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|
|
pCAS9id |
|
GAL1 (Cas9), pSNR52 (gRNA) |
URA3 |
Bertl
|
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018.
|
Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|
|
pCAS9cd |
|
TEF1 (Cas9), pSNR52 (gRNA) |
URA3 |
Bertl
|
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018.
|
Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|
|
pCAS9i_TRP |
|
GAL1 (Cas9), pSNR52 (gRNA) |
TRP1 |
Bertl
|
Bertl Lab Plasmids
(unpublished)
|
Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|
|
pCAS9id_TRP |
|
GAL1 (Cas9), pSNR52 (gRNA) |
TRP1 |
Bertl
|
Bertl Lab Plasmids
(unpublished)
|
Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
pCM188/p414-TEF1p-Cas9-CYC1t
|